栽培稻籼粳亚种间遗传差异及分子标记初步研究

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1、varieties，6typicalOryzasativassp．japonica,2cultivarsofOryzasativassp．javanicaand1cultivarsofuplandrice．Theresultindicatedthattherewere23variationalsiteswhichwere5．41％ofentirebasesand8informativesiteswhichwere1．82％ofentirebasesinOryzasatiwThelengthrangeofITSsequenceinOryzasativassp

2、．indicawasrangingfrom425to430bp：thepercentageofG+Ccontentvariedfrom74．25to75．59％．ThelengthrangeofITSsequenceinOryzasativassp．japonicawas425bpandthepercentageofG+Ccontentvariedfrom74．25to75．59％．ThephylogenetictreeconstructedonthebasisoftheITSsequencesshowedthatindicaandjaponicasubs

3、peciesofOryzasativaspeciesformedseparatecladesclearly，meanwhile，Oryzasativassp．jav幼icahavecloserrelationshipswithOryzasativassp．indica．ThestudyshowsthatthesequencesanalysisoftheinternaltranscribedspacerwasagoodtooltodiscriminatebetweensubspeciesofOryzasativaandwassignificantinther

4、esearchofOryzasativaevolution．2．TheanalysisoftherDNAintergenicspacerentiresequencesof13cultivarsinOryzasativa．Lwascarriedoutwith现rufipogonasoutgroup．Inthe13cultivars，therewere6typicalOryzasativassp．japonica,5typicalOryzasativassp．indica,2Oryzasativassp．javanica．Theresultindicatedt

5、hatthelengthrangeofIGSsequenceswasfrom2130to2145bp，thepercentageofG+Ccontentvariedfrom74．59％to75．29％，Vtherewere229variationallociwhichwere10．70％ofentirebasesand76informatirelociwhichwere3．51％ofentirebasesinOryzasativa．38indicaandjaponicaspecificlociwereexistedintheIGSsequencesofOr

6、yzasatJva,inwhichabout34oneswerecoveredaabout400bpsegmentlocatedintheupstreamofIGSsequence．TheindicaandjaponicasubspeciesofOryzasativ马aswellassomecloselyrelatedcultivars，canbedividedclearlybasedonthephylogenetictreeconstructedbyIGSsequences．Oryzasativassp。javanicahadaclosedrelatio

7、nshipwithjaponicasubspecies．3．Inordertoexploittheutilizationofheterosisbetweenricesubspecies，SSR，ISSR，SRAP，TRAPmarkerswereusedtoanalyzetherelationshipofinter—‘subspeciesandintra—subspeciesinoryzasativa．Theresultindicatedthatall4markerswerehighlycorrelated，and90indica—japonicaspeci

8、ficmarkersthatcouldhelpdifferenti