基因芯片检测hbv全基因组转染hepg2细胞株早期基因的表达

《基因芯片检测hbv全基因组转染hepg2细胞株早期基因的表达》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、中山大学博士学位论文ExpressionofInitialGenesinHepG2CellLineTransfectedwithFullLengthHBVGenomeStudiedbyGenechipMajor：InternalMedcineDoctor：LiYongweiSupervisor：ProfessorLiGangAbstractBackgoundThereare350millionpeoplesufferingfromHepatitisinfectedbyHepatitisBvirus(HBV)withah

2、ighriskofLivercirrhosis(LC)、HepatocellularCarcinoma(HCC)．20-30millionpeopleinChinaarechronichepatitisB(crm)．HBVinfectionisaseverehealthprobleminourcountry．Interferon(IFN)andsomenucleoside／tideanaloguesaleeffectivetotreatHBV,butwithlong-termtherapy,highpricesandi

3、ncreasingdrug-resistance．Tostudynewantiviralagentsaledifficultbeingshortageofknowledgeaboutthepathogenesisofthevirus．Thepathogenesisofthevirusisverycomplicatedincludinginnateandadaptimmuneofthehost，andhurtthehostcellsdirectly．AtthebeginningofHBVinvasion，thetissu

4、esandcells—immunesystem—effectors-immuneeffectivecellsofthehostintegratestoacomplicatedsynergismcooperatedwithallkindsofsignals．Inordertostudysuchhugeinformation，highthroughputgenechiptechnologyisveryappreciated．ThereportsaboutHBVstuiedbygenechipwereabouttheinfl

5、uenceofdifferentHBVproteinstothehost，treatmentwithsomedrugsandcomplicationsofhepatitisB．Butbifurcationexistsinmanyresults．Itrequiresfurtherstudysuch“stealth”virus．ThehumangenechipinAffymetrixCompanyistheperfectgenechipatthepresent．V中山大学博士学位论文Objective1Toobserveg

6、enechangesin2952celllinetransfectedwithfulllengthHBVgenometransiently，whichwasequivalentlytheacutephaseofHBVinfectioninhuman．TostudythepathogenesisofHBV．2Tostudyfurthertheimportantsignalingpathyway_tolllikereceptorsignalingandnaturalkillercellmediatedcytotoxicit

7、ysignalinginHBVinfection，andinhibitoryreceptor-KIR，activatereceptor-KLCandlumicanexpressesinsomecelllines，tostudytherelationofHBVandthem．MaterialsThefulllengthHBVgenome--pBlueScript··2952plasmidwasestablishedinourlaboratory,extractedtheplasmid，digestedbyLguIenzy

8、meandpurifiedthefragmentof3．2kilobases，transfectedHepG2cellline，examinedHBVRNAincells，hepatitisBenvelopantigenandhepatitisBsurfaceantigeninsupernatant．Thecells．named2