人参须超微粉成分的提取及抗肿瘤药理作用研究

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1、Abstractobjeetive：Thepurposeofthisstudyistostudytheisolation，purificationandidentificationofingredientsinultra-fineherbalparticlesofGinsengfiber,toinvestagatetheantitumoreffectsoftheingredientsinultra-fineherbalparticlesofGinsengfiberonthehumantumorcelllines，micebear

2、ingtumorsandtoexaminatetheacutetoxicity(LDso)，bloodserumbyadministrationofingredientsinultra-fineherbalparticlesofGinsengfiber,inordertoprovideexperimentaldateofpharmacodynamicsandtoxicologyforclinicalapplications。Methods：IIngredientsinultra-fineherbalparticlesofGins

3、engfiberwereobtainedthroughhotwaterextractmethods．2Thehumanlivercancel"cellIine(SK-HEP—1)，thehumanpoordifferentiatednasopharyngealcarcinomacellline(CNE一2Z)andthehumanleukemiacellline(HL-60)wereculturedinvitro，exposedtoingredientsinultra-fineherbalparticlesofGinsengfi

4、berwithfivedoses，therateofinhibitionandIC50wereobtainedbythemethodofMTTassay．4Onehunderdmicesweighing18—229(SPFGrade,halfmaleandhalffemal)weredividedinto10groups．ThemicebearingSarconl80(Sis0)，Ehrlieh(EAC)tumorswerepreparedinthefirstday．Thenthemicesweretreatedfor10day

5、sasfollows：({)NormalgroupWastreatedorallywithvehicleascontrol(Agroup,10rats，)；(室)positivecontrolgroupWastreatedbycyclophosphamide0．02眺g’d(Bgroup，10rats，)；③坷andWastreatedbyatdifferentdoseof5、2、1、O．5、0．259／kgrespectively．⑧～@wastreatedbyintegratedmedicine(2、1、0．259／kgin

6、gredientsinultra-fineherbalparticlesofGinsengfiber+0．029／kg’dcyclophosphamide)．Therateofinhibition(IR)ontumorsandtherateoflifeprolonging(PR)wereexaminatedrespectively．5CellcycleWasanalyzedbyflowcytometry(FCM)．46Contentsofmalondialdehyde(MDA)，nitricoxide(NO)，-SHandenz

7、ymeactivityofsuperoxidedismutase(SOD)，catalase(CAT)，Glutathioneperoxidase(GST)inbloodofmicebearingtumorswastestedbyphotometry．7Theacutetoxicityofingredientsinultra-fineherbalparticlesofGinseng丘beL[50％lethaldose(LD50)】WaStestedbyimprovingkarberassay．Bloodserumalaninea

8、minotransferase(ALT)，asparticaicdaminotransferase(AST)activity,andCreweremeasured．Results：lAnti-tumoreffectsofingredientsinultra-fi