半夏的种质资源鉴定-研究及加工方法对其品质的影响

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1、treatmenttokillenzymes，intherolesofpolyphenoloxidase，willoccurbrowningevendecay．Forpreventingthisphenomenon，someonejoinedRhizomaPinelliaewithonekindofantiputrefactivecalledthepreservativesodiumsulfatewhichiseasytocausecancer．IthasseriouslyaffectedthequalityofRhizomaPinellia

2、e．Inthispaper,weusedtheconventionalmethodsintheChinesePharmacopoeia，foundthatsomesamplesofRhizomaPinelliaecontainsodiumpyrosulfiteindeed．ThecomponentofRhizomaPinelliaewithlow．temperatureandnaturallydriedeasilychanges，whichalsoeasilydecompose．That’Sbecauseofthedamageofpolyph

3、enoloxidaseinit．ItisrevealedthattheprocessingmethodtoRhizomaPinelliaehasaverycloserelationshiptoitsqualityandstorage．Inordertoconfirmingtheaboveexperimentalresult，weanalysisedthewater-solublecomponentscontainedinRhizomaPinelliae．WithHPLCchromatography，thesamplesofRhizomaPin

4、elliaeprocessedbydifferentmethodshavebeendetermined．ThemobilephaseiSmethanol—watersystem,examinationwave．1ength210nm，thecolumntemperature30℃，flowvelocity1．0ml／min．Theexperimentalresultsshowedthat：ComparedwiththesamplesofRhizomaPinelliaewhichdried2hby70。Cthenbakewithlowtempe

5、ratureandfreshRhizomaPinelliaejustexcavated，thewater-solublechemicalconstituentsofthesamplesofRhizomaPinelliaewithoutproperheattokilltheenzymeandfreshRhizomaPinelliaeinthegerminationdecreasedsignificantly．Andwhentherearecorrectsampleandreferencesubstance，wecanalsoanalysisth

6、efingerprintofwater-solublechemicalinRhizomaPinelliaefurthermorewiththeHPLCchromatographyestablishedinthispaper．Finally,usingthemethodinducingdormancyofthemicewithsubliminalandsuprathresholddoseofsodiumpentobarbitaladdingethylacetateorn-butanolpartofRhizomaPinelliae(athight

7、emperaturestokillenzyme)ethanolextract，wecomparedthesedativeandhypnoticactofthesetwopart．Theresultsshowedthat：theethylalcoholpartofthetotalextract，comparedwiththen-butanolpart．hasbettersedativeandhypnoticeffectsofsedativeandhypnotic．Theactiveingredientsshouldbeinitsethylace

8、tatepartofthetotalextract．Keyword：Rhizomapinelliae；Isoelectrofocusing；GC；HPLC；Seda