中链三酰甘油对力竭游泳小鼠抗疲劳的作用研究

《中链三酰甘油对力竭游泳小鼠抗疲劳的作用研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、中链三酰甘油对力竭游泳小鼠抗疲劳的作用研究lipidmetabolisminblood，liverandgastrocnemiusmuscle，andreducingtheliveroxidativelevel．Byusingthereal-timequantitativePCRtechnology,theregulativefunctionofMCTforPPAR-aandPGC-laexpressionwasalsofurtherdiscussed．Besides，theexpressionofPPAR-ainliverandAMPK-aingastrocnem

2、iusmusclewereassessedtogetherbyusingtheWesternblottechnology．Inaword，thoseresearchesprovidenewexperimentalevidencesandtheoreticalsupportsforthealleviating-fatigueeffectofMCTinenduranceexercises．Objeetives1．Toaccessthealleviating—fatigueeffectofMCTinenduranceexercises．2．Toresearchthemec

3、hanismofMCTalleviatingphy7siealfatigue．3．ToprovidescientificevidencefortherelationshipbetweenMCTandphysicalfatigue．Methods1．ExperimentalanimalsandGroupsMaleICRmiceinthesestudieswereprovidedbyExperimentalAnimalCenterofSecondMilitaryMedicalUniversity．First，themicewerehousedforanadaptivef

4、eedingoflweekbeforetheexperiment．Second，theywererandomizedintotwelveGroupsbasedontheirbedyweight，with6miceineachgroup．Third，themiceweremarkedfrom1t06iIlonecage．Altogether,therearesixbigGroups(12miceineachgroup)：untreatedcontrolGroups(g1andg12)，untreatedswimminggroup(921and922)，MCT6％gro

5、up(931and932)，MCTl8％group(941and942)，MCT30％group(951and952)，andMCT44．4％group(961and962)．Eachmousewasgiven4．59／dexperimentaldiet，andhadfleeaccesstowater．AttheSalTletime，allGroupsweresubjectedtounloadedswimmingtrainingonMonday,WednesdayandFridayinoneweek．Themiceweretrainedfor20rainwithsu

6、bsequentincreaseof10mineachtimeuntilitreached40min．Theexperimentalperiodslast3weeks．Untreatedcontrolgroup(UCG)anduntreatedswimminggroupfoso)werefedwithD12450Bdiet，withoutcontainingMCT．Theprotein,carbohydrateandfatcontentscorrespondedto20％，70％and10％ofmetabolizableenergy．BothGroupshadswi

7、mmingtrainingduringtheexperimentalperiod．UCGwereconsideredasthebefore-exercisesstatecomparedwithUSGAllMCTGroupsweregiventhemodifiedD12450BdietssupplementeddifferentdosagesofMCT．Amongallthecomponents，lardWassubstitutedinportionbyMCT,whichaccountedfor6％，18％，30％and44．4％ofthetotal缸．AⅡ·5．