一体化管盖基因芯片检测系统(光学与控制)研制

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1、AbstractThemicroarraytechnique,ahigh—throughputdetectionmethod，hasbeenwidelyapplied．ThecoretechniqueinvolvedingenearraysisthereverseoftheSouthernblottechnique．Themicroarrayprogressisquiteeasytocauseunstableresultbecauseofitscomplexprocessandmultistage．Themicroarray-in-a-tubesystemisaseale

2、dsystemforthemicroarraytechnique．Inthesystem，anoligonucleotidemicroarrayisarrangedontheinnersurfaceofaspecialdesignedofEppendorfcap、析thaflatopticallytransparentwindow．Aninnervesselforthestorageofthehybridizationsolutionisplacedinthesealtube．Themicroarraysystemsimplifythecomplexityandperfo

3、rmnonecontaminationmicroarraydetectionbecauseoftheencapsulatedsystem．However,commercialdetectionsystemisn’tsuitableforthemicroarraydetectionsystem，becaussethemicroarray-in-a-tubeisastrangedevicecomparedwiththestandardglassmicroarrayslide．Asaresult,adetectionsystemwithanopticalsystem,allau

4、tomaticscanningsystemhasbeendevelopedforthemicroarray-in—a-tubedetectioninthelaboratory．TheopticalsystemWasdevelopedbymeansoffluorescencemicroscopeandNikonD90camera．LaserWasselectedasilluminationsourceandalaserbeamexpansion&energyhomogenizationsystemWasdesigned．Automaticscanningsystemcons

5、istsofacontrolcircuitanda3-Dmobileplatformtoperformscandetectionofmultimicroarray-in—a—tubes．TMS320F2812DigitalSignalProcessorwhichishighlyintegrated，high—performancesolutionsfordemandingcontrolapplicationsWasselectedashostcontrollerinthecircuit,TheCypressSemiconductorCY7C68013A-128isused

6、8．SusbinterfacechiptodealswimcommunicationbetweenPCandF2812inthesystem．The3-Dmobileplatform’Stransmissionmechanismconsistsofballserews，linearguidetracksandslides．Testresultsindicatethattheopticalsystemandautomaticscanningsystemarecapableofsatisfydetectionrequirements．Comparetootherchipsca

7、nner,advantagesofourdetectionsystemaremuchevident,foritislow-cost，small—size，lowpower，portableandeasytooperate．KEYWORDS：microarray；fluorescenceimagingdetection；electroniccontrol；mobileplatformII目录摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯IAbstract⋯⋯⋯⋯⋯⋯⋯⋯