α硫辛酸对高脂饮食去卵巢肥胖大鼠海马炎症反应与bdnf水平的影响

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1、山东大学硕士学位论文Effectofa．1ipoicacidoninflammationandthelevelofBDNFinhippocampusfromobeseratsinducedbyMajorovariectomyandahigh—fatdietInternalMedicine(EndocrinologyandMetabolism)MastercandidateYulianMiaoSupervisorProf．XiaoliZhangobjectiveABSTRACTObesity，whichisastatecharacterized谢thchronicinfla

2、mmation，notonlyincreasestherisksofinsulinresistance，metabolicsyndromeandcardiovasculardisease，butalsocontributetocognitivedecline．IthadbeenconfirmedthatQ-lipoicacid(a-LA)hadtheabilityofamelioratinglipidabnormalitiesandimprovinginsulinsensitivityinobeserats，whichwereinducedbyconsumptionofa

3、high-fatdiet，throughantioxdativeandanti—inflammationeffects．Moreover，Ct·LAhasbeenshownananti-inflammatoryandneruroprotectivefunctiononAlzheimer’Sdisease．Inpresentstudy,ananimalmodeltomimickthepostmenopausalandhyperlipidemicsituationwasinducedbytheassociationofovariectomy、析thahigh-fatdiet，

4、andthentoexploretheeffectsofovariectomyplushigh-fatdietoninsulinresistanceinserum，inflammatoryreactionandthelevelofBDNFinhippocampus．andtoinvestigatetheeffectsofa—LAontheirexpressions．MaterialsandMethods1．AnimalsFortyfemaleWistarrats(three-monthold)wererandomlydividedintofourgroups(n=10pe

5、rgroup)：sham-operationwithnormaldiet(SHAM+ND)；sham-operationwithhigh-fatdiet(SHAM+HFD)；ovariectomywithhigh—fatdiet(OVX+HFD)；ovariectomyplushigh-fatdiettreatedwith4山东大学硕士学位论文cc—LA(OVX+HFD+a—LA)．ExceptforSHAMgroups，otherswereunderwentbilateralovariectomy，andthenratsweredosed诵mQ—LAsolution(2

6、00mg·kgq-d‘1)orsodiumcarboxymethytcellulosesolutionbygavageoncedallyfor12weeks，andbodyweightsofrotsweremeasuredonceaweek．2．BloodandhippocampussamplepreparationAfter12weeks，bloodWascollectedfromfemoralartery．Bloodsampleswerecentrifugedat3000rpmat4"Cfor15min，serumwasseparatedandstoredat一80。

7、Cuntilassayed；Ratsineachgroup(n=8-10ineachgroup)wereterminatedbydecapitationandthebrainswererapidlyremoved，thenthebilateralhippocampiweredissectedontheiceandimmediatelystoredinliquidnitrogen．3．MethodsofmeasurementTheserumconcentrationsof1713-estradiol(E2)weredetecte