skov3与epcs共培养体系建立与vegf抗体对epcs成管的影响

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1、第三军医大学硕士学位论文缩略语表英文缩写英文全名中文译名AMFAutocrinemotilityfactor自分泌运动因子BCABicinchoninicaciddisodium二喹啉甲酸BSABovineserumalbumin牛血清白蛋白cDNAComplementaryDNA互补脱氧核糖核酸DAPI4,6-diamidino-2-phenylindole二脒基苯基吲哚DEPCDiethylpyrocarbonate二乙基焦碳酸酯DiI-ac-LDLDiIlabeled-acetylatedlow-羰花青标记乙酰densitylipoprotein化低密度脂蛋白DM

2、EMDulbecco’smodifiedEaglemediumDulbecc改良Eagle培养基EDTAEnthlenediaminetetraaceticacid乙二胺四乙酸二钠EGMEndothelialgrowthmedium内皮生长培养基EPCsEndothelialprogenitorcells内皮祖细胞FBSFetalbovineserum胎牛血清FITC-UEA-1Fluoresceinisothiocyanate-labeled异硫氰酸荧光素标记的ulexeuropaeusagglutinin1荆豆凝集素1HIF-1Hypoxiainduciblefac

3、tor-1缺氧诱导因子-1MNCMononuclearcell单个核细胞MMPsMatrixmetalloproteinases基质金属蛋白酶mRNAMessengerribonucleicacid信使核糖核酸PBSPhosphatebufferedsaline磷酸盐缓冲液RT-qPCRReal-timequantitativepolymerase实时荧光定量chainreaction聚合酶链反应SDS-PAGESodiumdodecylsulfatepoly-十二烷基磺酸钠-烯酰acrylamidegelelectrophoresis胺凝胶电泳TLRToll-like

4、receptorToll样受体VEGFVascularendothelialgrowthfactor血管内皮生长因子VEGFR-2Vascularendothelialgrowthfactorreceptor2血管内皮生长因子受体2VE-cadherinVascularendothelialcadherin血管内皮钙粘蛋白1第三军医大学硕士学位论文TheestablishmentofSKOV3co-culturewithEPCsandVEGF-antibody’sinfluenceonEPCsintubeformationAbstractBackground：Ovari

5、ancancerisoneofcommonmalignanttumoroffemalereproductiveorgans,themaintreatmentissurgery,butthe5-yearsurvivalrateofonly25%-30%.Themainreasonofhighmortalityrateisthemetastatisandtherecurrenceofovariancancer,thekeyfactorofitisthetumorangiogenesis.CurrentlybesidestheacceptedVEGF(vascularendo

6、thelialgrowthfactor)isthemainfactortopromotetumorangiogenesis,Endothelialcellsfromvarioussources(suchasvascularendothelialcellsfromtumor,endothelialcellsincirculation,etc.)arealsoimportantfactorstopromotetumorangiogenesis.EPCs(endothelialprogenitorcells)istheprecursorcellsofmatureendothe

7、lialcells,candifferentiateintoendothelialcellsandparticipateinangiogenesis,soEPCsisalsoaimportantfactoroftumorangiogenesis,sothestudiesofEPCshaveimportantsignificancetotumorangiogenesisandinhibition.AsthefeaturesofEPCs’surfacemarkersanddifferentiation,itispossibletocanred