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1、湖南农业大学学报(自然科学版)2017,43(6):657–661.DOI:10.13331/j.cnki.jhau.2017.06.015JournalofHunanAgriculturalUniversity(NaturalSciences)投稿网址：http://xb.hunau.edu.cn中草药抑制剂对中国对虾致病菌哈维氏弧菌的抑制效果*樊英，盖春蕾，王晓璐，许拉，刁菁，于晓清，叶海斌(山东省海洋生物研究院，山东青岛266104)摘要：用抑菌圈法和微量二倍稀释法研究金银花、车前草等16种中草药提
2、取液和纳米锌、纳米银对中国对虾来源病原菌——哈维氏弧菌TCBS–G的抑制效果。结果表明：纳米锌对TCBS–G产生的透明抑菌圈直径达35mm，地锦的透明抑菌圈直径约25mm；采用96孔板倍数稀释法测定最小抑菌浓度(MIC)，纳米锌、地锦的最小抑菌浓度均为12.5mg/mL，纳米银的达6.25mg/mL；采用MTT比色法检测不同抑制剂对哈氏弧菌生物膜形成的影响，纳米锌、纳米银对TCBS–G生物膜形成具有明显的抑制作用，药液质量浓度大于3.125mg/mL时具有显著差异(P<0.05)；黄芪、地锦药液质量浓度大
3、于12.5mg/mL时抑制作用显著(P<0.05)。普通药敏试验结果显示，该菌株对头孢他啶、庆大霉素、复方新诺明等药物极其敏感，抑菌圈直径达20mm以上，对头孢氨苄、四环素等均不敏感。关键词：中国对虾；哈维氏弧菌；中草药；纳米锌；纳米银；敏感性+中图分类号：S945.19文献标志码：A文章编号：1007−1032(2017)06−0657−05EffectsofdifferentinhibitorsonVibrioharveyifromFenneropenaeuschinensisFANYing,GAIC
4、hunlei,WANGXiaolu,XULa,DIAOJing,YUXiaoqing,YEHaibin(MarineBiologyInstituteofShandongProvince,Qingdao266104,China)Abstract:TheinhibitoryeffectsofLonicerajaponica,Plantagodepressaandother16herbalextractsandnano–zinc,nano–silveronVibrioharveyi(TCBS–G)fromFen
5、neropenaeuschinensiswerestudiedbymeansofdiscagardiffusionandmicrodoubledilutionmethods.Theresultsshowedthatnano–zinchadastronginhibitoryeffectonTCBS–Gwiththediameterof35mminhibitionzone,followedbyHerbaeuphorbiaewiththediameterof25mm.TheMICofnano–zincandHe
6、rbaeuphorbiaedetectedon96wellplatesviamultipledilutionmethodwas12.5mg/mL,thevaluewas6.25mg/mLforthatofnano–silver.FromthetestofdifferentinhibitorsonbiofilmformationofVibrioharveyibymeanofMTTassay,wefoundthattheinhibitoryeffectofnano–zincontheformationofV.
7、harveyibiofilmwassignificant,especiallywhenthesolutionconcentrationwasabove3.125mg/mL(P<0.05),andtherewerealsosignificanteffectsforAstragalusmembranaceusandHerbaeuphorbiae,whentheirconcentrationwereabove12.5mg/mL(P<0.05).Theresultfromsusceptibilitytestofg
8、eneraldrugsshowedthattheTCBS–GwasverysensitivetoCeftazidime,Gentamicin,compoundSulfamethoxazolewithlessthan20mmdiameterofinhibitionzone,however,itwasnotsensitivetoCephalosporins,Tetracyclineetc.Keywords:Fenneropenae
