pokemon在肾癌中的表达及其临床意义

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1、大连医科大学硕士学位论文差异，然后对mRNA和蛋白表达情况进行相关性分析。采用SPSSl3．0软件对数据进行统计学处理，所得计量资料采用随机区组方差分析，P<0．05为差异有统计学意义。结果：(1)肾癌组织标本，Westemblot方法和RT-PCR方法结果显示肾癌组织中pokemon在蛋白和mRNA水平的表达量明显高于癌旁肾组织中的表达量，差异有统计学意义。(2)肾癌组织标本，低分化组中pokemon在蛋白和mRNA水平的表达高于中、高分化组，差异有统计学意义；转移组中pokemon在蛋白和mRNA水平的表达高于未转移组，差异有统计学意义；Pokemon在蛋白和

2、mRNA水平的表达与患者性别、年龄、肿瘤大小及TNM分期无明显相关。(3)细胞标本中，人肾透明细胞癌细胞系769．P中pokemon在蛋白和mRNA水平的表达明显高于肾近端小管上皮细胞系HK．2，差异有统计学意义；应用Spearman相关性分析蛋白和mRNA表达情况，显示两个在人。肾透明细胞癌细胞系769．P、肾近端小管上皮细胞系HK。2的表达呈正相关，r=0．457。结论：1．肾癌组织和细胞中pokemon在蛋白和mRNA水平的表达明显高于正常肾细胞，提示pokemon与肾癌的发生发展相关性，它可能在肾癌的发生演变中起重要作用。2．Pokenmon的表达与肾癌临

3、床病理分级、是否转移有关，而与性别、年龄、肿瘤大小、TNM分期无明显相关性。3．此结果与pokemon在人类肺癌、前列腺癌、结肠癌、淋巴瘤和膀胱癌等多种肿瘤中均有过度表达一致，进一步证明pokemon对包括肾癌在内的多种肿瘤有重要的调控作用。关键词：肾癌pokemonWesternblotRT-PCR2Ex[andclinicalsignificanceofpokemon‘aExpressionandclinicalsi2nicanceofookemonInrena—lCarClnomaMasterdegreecandidate：SongyujianSupervi

4、sor：ProfessorHezhongzhouMajor：SurgeryAbstractBackgroundandobjeetives：Renalcarcinomaisacommonmalignanttumororiginatingfromtheurinarytubularepitheliaofrenalparenchyma．Amongallurinarytumors，itsmorbidityrateranksonlyafterbladdercancer．RenalcarcinomawillOccurinanypartofrenalparenchymaandac

5、countsfor3％ofadultpatientsofmalignanttumors．Themorbidityrateofrenalcancerisstillontheriseinrecentyears．PokemonisamemberofthetranscriptioninhibitorfamilyPOK．TheproductsencodedbyPokemonhaveBTBdomainandzincfinger,andthusPokemonplaysanimportantroleintheoccurrenceanddevelopmentoftumors．Pok

6、emonisinvolvednotonlyintheregulationofcellcycleandcellapoptosis，butalsoactsasacentralregulatoryfactoroftheanti—oncogene—alterativereadingflame(ARF)．Studiesshowthatpokemonisoverexpressedinhumanlungcancer,prostatecancer,coloncancer,lymphoma，andbladdercancer,andthatpokemoncailaffectsomei

7、mportantcharacteristicsoftumorcellsandmaybenecessaryintheinductionofcancersbyothercancergenes．Theobjectivesofthispaperaretoinvestigatetheexpressionsofpokemoninrenalcarcinomaattissueandcelllevels，andtoprovideafoundationforfurtherstudyontheassociationofpokemonwiththeoccurrenceanddevelop

8、mento