egr1特异脱氧核酶对大鼠颈总动脉球囊损伤术后pai1和tf表达的影响

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1、·英文论文摘要·EffectsofDNAEnzymeTargetingEgr-1onTheExpressionofPAl--landTFafterBalloonInjuryofRatCommonCarotidArteryAbstractToinvestigatetheeffectsofDNAenzymetargetingEgr-lmRNA(ED5)onPAI．-1andTFaftervascularballooninjuryandthemechanismofinhibitingneointimalhyperplasia．Methodsandme

2、thod1．SynthesisofED5WassynthesizedbyShanghaiBioasiaandthebasesequenceWas5'-．C．．．．C．．．．G．．．．C．．．．T．．．．G．．．．．C．．．．C．．．．A．——GGCTAGCTACAACGACCCGGGACGT-3’．ED5wasmodifiedbyt1_lioxoXatthe3’endand5’end，andwaslabeledwithfluoresceinisothiocyanate(FITC)atthe5'endtoobservethedistributio

3、nofgenesaftertransfectionunderfluorescencemicroscope．2．EstabifishmentofratcarotidinjurymodelandexperimentalgroupmgThepresentexperimentswerereviewedandapprovedbytheCommitteeonEthicsonAnimatlExperiments,．ChinaMedicalUniversity,accordingtotheGuidelinesoftheAmericanPhysiologicSo

4、ciety．ThisstudyWaspcrforomedatChinaMedicalUniversity．Ninty-sixWistarmaleratsweighing350·-4009wererandomlydividedintofourgroups：thesham-group，,theMgcl2group，．theFuGene6‘groupandED59roup·Thesham．group，,theMgcl2groupandtheFuGene6groupservedascontrolgroups·Theratswereanesthetize

5、dwith10％hydronaldehyde(300mg／kg)．Thanwhich6werecutinthemiddleoftheneckandtheleftcommonandexternalcarotidexposedandstoppedfromthebleedingatnearordistalendbytheserrefine．A2-frenchFogartyballooncatheterwasintroducedthroughanarteriotomytotheexternalcarotid，advancedintothecommonc

6、arotid，inflatedtogenerateresistanceandwithdrawthreetimesinamannerthathadbeendescribed．Afterremovalofthecatheter,a200I_tLsolution(at4"C)containingFITC-labeledED5500I．tg，transfectionreagentFuGene6301Aand170I．tLlmmol／LMgcl2wasinjectedlocallyintoinjuredvascularsubsectionfor20min

7、utesinED5group．Theintimaldenudeationandgreenfluorescentexcitedby470-490nmopticalcouldbeseenintheintimalandmediallayers011the24‘hhourafterdeliveryofFITClabeledDNAenzyme(ED5)bythefluorescencemicroscope．A200I_tLsolution(at4。C)conatiningtransfectionreagentFuGene630rtLand170I-tLl

8、mmol／LMgcl2wasinjectedlocallyintoinjuredvascularsubsectionandincubatedfor20