三种化学方法制备脱细胞神经的比较及新型神经支架材料的制备

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1、ThecomparisonofthreekindschemicalacellularmethodstoprepareacellularnerveandpreparationofanewnervescaffoldABSTRACTTherearetwoPartoftheexperiment.PartOne:ThecomparisonofthreekindschemicalacellularmethodstoprepareacellularnerveObjectThroughthecomparisonofthreekindschemicalacellularmethodstopre

2、pareacellularnervetochoosethemostappropriatetechniquetoprepareacellularnerve.Methods1.TodealwithsciaticnerveofSDratwithlysophosphatidylcholinemethod,trinitrotol-ueneandSodiumdeoxycholatesolutiontoinroomtemperaturealternatively,aswellassodiumazide,trinitrotolueneandsodiumdeoxycholatesolution

3、tointurntopreparedecellnerveseparately.2.Toestimatethemorphologicalofthethreekindsofdecellmaterialbyolefinslices.Third,performcytotoxicityandsubcutaneousimplanttesttothematerials.Results1.NochangesoccurredtothediametersandlengthsofthethreeclassesofdecellnerveScaffolds,softtexture,ductile,iv

4、orywhiteandtranslucenthavebeenexisted.Theirelasticitywasaslightlypoorerandthetenacitywasalittlebetterthanfreshnervestructures,theductibilitywasbetterafterbechemicalextractedandcandoconventionalnerveanastomosiswithoutstrain.2.2.HEStainingthedissolvedscrapsofaxoncellcanbeseeninthecrosscutpiec

5、esobtainedbyDumontmethod,whichmeansthecellshaven’tbeenextractedthoroughly.Perineuriumandoutermembranebedyedredappearedaroundthe6irregularroundhollowsformedbytheendoneuriumwhichhasbeendyedred.Endoneuriumbedyedredarrangedlikeswaveontheverticalcutpieces,aswellasthecolumnar-tubelikeinterspacewh

6、ichformedbythedisappearedaxonandmyelinsheat.BigspacesappearedinthecavityoftheendoneuriumbedyedredonthecrosscutandverticalcutpiecesacquiredbySondellmethod.WhiletheendoneuriumtubesarrangeduniformlyonthepiecesobtainedbyHaasemethodandnobigspacesexisted.3.LamininimmunohistochemicalstainingTheSCB

7、MdyedyellowaroundtheemptyendoneuriumtubebekeptonthepiecesacquiredbyDumontmethod.NormalneuromechanismdisappearedontheveticalcutpieceswhiletheSCBMdyedyellowhasbeenreserved,arrayedlikeswave.TheneuromechanismmyelinsheathdisappearedonthecrosscutpiecesgotbySon