mir-29a在动脉粥样硬化发生发展中的作用及其分子机制

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1、英文摘要Theresultsofhighresolutionultrasonographyshowedthatplaqueandthrombosiscouldbedetectedbyhighresolutionultrasonography．Allplaqueswerecharacterizedasfibrofatty．TherearenodifferencesbetweenAP,ADandAMgroup．4miR-29apromotetheneointimalformationinatheroscl

2、eroticApoE’7’miceMorphologyanalysisshowedthemarkedneointimalformationafterthehi曲-fatdiet．NeointimalformationandplaqueswerealldetectedinAGAPandAMgroup．TherearenodifferencesinAGandAPgroup．However,comparedwithAPgroup，neointimalformationwasincreasedinAMgrou

3、p，whichsuggestingmiR-29amayincreaseneointimalformationbypromotingtheproliferationofVSMCs．5miR-29adidnotaffectthelipiddepositioninatheroscleroticApoE叫‘miceTheeffectofmiR-29aonplaqueextensionintheaorticoriginwasevaluatedbyanalyzinglipiddepositiononoilredO

4、stainedtissuesectionsoftheaorticroot．ThemeanareasoflipiddepositionintheaorticrootwereincreasedinAP,AGandAMgroup．TherearenodifferencesbetweenAP,ADandAMgroup，suggestingmiR一29ahadnoeffectonlipiddeposition6miR-29aincreasedthecollagencontentofplaquesinathero

5、scleroticApoE‘

6、。miceWhereaslipiddepositsmakeplaquesmorepronetorupture，collagenfbersstabilizeplaques．Therefore，weassessedtheeffectofmiR-29aonthecompositionofplaquesintheartery．Theareasoflipiddepositsandtheareasofcollagenfiberswithinaplaqueweredeterminedo

7、noilredOandadjacentSiriusstainedtissuesections，respectivelyandtheratiowascalculated．Thecollagen／lipidratioofmiR-29atreatedmice(AMgroup)WasmorethantwiceashighasinAPgroup，supportingtheobservationthatthemiR-29ahasaplaquestabilizingeffect．7miR-29adidnotaffe

8、cttheinfiltrationofplaqueswithinflammatorycells5英文摘要Becauseinadditiontotheplaquecomposition，inflammationisthoughttohaveagreatimpactonthevulnerabilityofplaques，weassessedthedegreeofinflammationofplaquesinmiR．29atreatedmiceoftheatheroscleroticmodel．TodoSO

9、，tissuesectionsoftheaorticoriginwereimmunostainedwithspecificmarkersformacrophages(F4／80)．Hence，miR-29adidnotaffectthemeanrelativeinfiltrationofplaqueswithmacrophageintheaorticroot．1here．●一‘rn‘arenodifferencesbetweenAP,ADandAMgro