鸡传染性支气管炎重组鸡痘疫苗免疫效力的研究

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1、AbstractAvianinfectiousbmnchitisispreValentinancoun仃ieswithanintensiVepoulnyindusnyTheVirusisbelongtogroup3coronaviⅢs．AVianinfectiolIsbmnchitis雒enuatedvaccinesplay柏importantr01einthecontrolofIBVaUovcrt11eworId．M锄ycommerc．a1attenuatedvaccinesderiVedfromlocalIBVlieldjs01ateshavebee

2、nusedtocontroIIBVinChinabesidesMaSsachusettsserotypeVaccinesH120柏dH52thatwereimported．Butbecauseofdi髓rentgenotypes，IBoutbreaI(s靠equentlyinChinainVacci舱dnocks．1tissuggestedthatfowlpoxvirus如avectorissafty粕de币cient．wehaveconstructedrFPV-S1·IFNY锄drFPv-S1successfullyInthissnJdytheexpe

3、rimentisaboutlmmunologicaIE币cacy，containingMinim啪immllfledose，sa】fly，genetics缸IbI“ty锄dstoragecondition．rFPv-sl-IFN叶甜drFPv．s1、veresuccessivcpaSsaged30inchickenemb驴肋robl鹊t．TheSgene卸dIFN丫gene疗0mp般age15、20、25柚d30wereanlplinedbyp01ymerasechainreaction(PcR)柚dsequenced．ProteinslandIFN丫e

4、xpressedbyrFPv-s1-IFN丫锄drFPv-slwereidemmedbyIndirec“mmunonuorescence髂say．Theresul乜showthattherewerenochangesinthearninoacidsequences．The15th，20th，25th，30thofrccombinamfowlpoxVirusexpressedoVertlybyIndirect．immunonuorescence硒sayrFPV．sl-IFN丫andrFPv_slhas900dgeneticalstabil吼l·wcek—o

5、ldSPFchickenswereinoculaledwithrFPv_S1．IFNyrFPv-S1(1．0x105PFU／chicken)，theresultsshowthattherecombinantfowIpoxvaccinesweresaf吼onehundred4-week-oldSPFchickenswererandomlydividedinto4groups，a王1djnoculatedwithrFPV·S1-lFNy，rFPV—sl，H120andPBS(contr01)．Fourweekspost-vaccination，allchic

6、kenswerecha【lengedwithIBVLX4stmin．Theresultsshowedthat13Outof22chickensimmunizedwithrFPv-S1-IFNywereprotected，andtheprotectionratesingroupsimmunizedwithrFPV-S1orlivelBVaccinealonewere18／24and17／22．Afterchallenge，chickensofrFPv-S1-IFNyhadashortertimeperiodofVirussheddingfromrespir

7、atorytractandtheViralantigenpersitinginkidney，incomparisonwithrFPV_S1andH120group．SlightpathologicalnndingscouIdbeseeninliveLspleen，kidney，lungandtrachealinH120鲫drFPVSl．andrFPv-Sl-IFNyperfonnedevenbenerthanotherS，pathologicalchaflgescouldonlybeseenat16dayspost-chanllenge，whichwas

8、4daysshorterthanthatofothergroups．A

9、lchi