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1、AbstractSulfur-oxidesfromcombustionofcoalhavecausedaseriousenvironmentpollutionproblem.Moreandmorestringentenvironmentpollutecontrolstandardsdemanddeepdesulfurizationtechnology.Comparedtotraditionaltechnologies,biodesulfurizationtechnologyhassomeadvantagessuchasthemildre
2、actionconditions,lessequipmentandoperationalcosts,andselectiveremovalofsulfurfromcoal.Asaresultbiodesulfurizationtechnologywillbeaefficientdeepdesulfurizationtechnology.#AnewstrainnamedYBS2thatcandegradedibenzothiophene(DBT)into2-hydroxybyphenyl(2-HBP)wasisolatedfromthes
3、ewageofHohhotrefinery.ThestrainwasidentifiedasMicrobacteriumbasedonmorphologicalobservations,aswellasphysiologicalandbiochemicaltests.Theoptimalcarbonsource(glycerol)andnitrogensource(ammoniumchloride)wereobtainedbysingle-factortestsandorthogonaltest.Theresultsofsingle-f
4、actortestsandorthogonaltestalsoshowedthattheoptimaloperationalconditionswereasfollows:initialpH,7.5,temperature,30℃,concentrationofDBT,0.1mM,inoculumsize(%),10%,brothcontent,200mLbrothper500mLflask.Undertheoptimalconditions,thedegradativeratioofDBTreached64%in4days.Apint
5、-sizeddesulfurizationbioreactorwasdesignedandthedesulfurizationactivity#ofYBS2fororganicsulfurinhigh-sulphurcoalwasstudiedinthereactor.Thestrainremoved70.45%oforgano-sulfurand45.05%oftotalsulfurfromcrudecoalin7days.Theeffectoftempertrue,initialpH,coalgranularity,coalslur
6、ryconcentration,stirringspeedandventilationrateondesulfurizationefficiencywereobserved.Theresultsshowedthattheoptimalconditionswereasfollows:temperature,35℃,initialpH,7.5,coalgranularity,150,coalslurryconcentration,10%,stirringspeed,160r/min,ventilationrate,2.0L/min.Atth
7、esametime,anewstrainnamedDDTwhichcanremovesulfurousironorefromcoalwasisolatedfromthecollieryinDatongcityandidentifiedasThiobacillus.Inthebioreactor,strainDDTremoved58.5%ofsulfurousironoreand33.5%oftotalsulfurin14days.Studiesontheinfluenceoftempertrue,initialpH,coalgranul
8、arity,coalslurryconcentration,stirringspeedandventilationrateondesulfurizationefficiencyshowedtheoptima