宫内缺氧对幼年大鼠海马ca3区神经元与学习记忆能力的影响机制

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1、宫内缺氧对幼年大鼠海马CA3区神经元与学习i．E忆能力的影8州u羽uLg莉2；一慨弧《1l《凯§‰轴l在400倍下用网络版数码显微镜DMBA200对海马CA3区拍照。IPP6．0软件图像分析，记录各免疫组化阳性细胞的积分光密度(integralopticaldensity,IOD)值。统计学分析：两组之间比较用独立样本t检验。结果：Morris水迷宫实验显示缺氧组幼年大鼠探查测试时间(T1=18．474-4．41s)较对照组幼年大鼠探查测试时间(T1=24．384-4．89s)明显缩短，并且缺氧组幼年大鼠对位探查测试时间(T2=15．384-6．

2、10S)较对照组幼年大鼠对位探查测试时间(T2=22．044-5．57s)亦明显缩短，差异均有统计学意义(尸

3、均数(6．244-0．52)大，且差异有统计学意义(尸

4、lativehumidity：75％一80％)startingfromday14ofpregnancyfor2h／dfor5consecutivedays(gestational14—18thdays)toestablishafetalratintrauterinehypoxiamodel．Controlgrouplackedthehypoxicconditions．Afterbirth，twoneonatalratswereselectedrandomlyfromeachlitterandfedfor30days(inordertobalanc

5、etheneonatalratsnumberamonglitters，theremainingneonatalratswerekilled)．The30一day-oldjuvenileratsweretestedbyMorriswatermazefor1days，searchingtime(T1)inthetargetquadrantinprobetrailofwatermazeexperimentandsearchingtime(T2)inthetargetquadrantinreversalprobetrailofwatermazeexper

6、imentwererecorded．Andthenthesejuvenileratswereperfusedandfixedwithformaldehyde(40g／L)，afterthatbraintissueswereremoved，dehydrated，paraffin—embeddedandsectionedseriallyforhippocampalcoronalsections(thickness：5“m)toproceedwithimmunohistochemistrydetection．Insection3immunohistoc

7、hemistrydetection，amonoclonalanti—AChEantibody(1：200dilution)，amonoclonalanti-SYNantibody(1：150dilution)andapolyclonalanti—Jmjd6antibody(1：80dilution)wereused．Specificstepsofdetectioninthisexperimentwereasfollows：①Conventionaldewaxinginwater．②Preconditionedthesectionsaccordin

8、gtothespecialrequirementsoftheprimaryantibodies．⑧Incubatedfor10minin