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ID:32229064
大小:1.50 MB
页数:36页
时间:2019-02-01
《rala、rhoc在非小细胞肺癌组织中的表达与意义》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、RaIARhoC在非小细胞肺癌组织中的表达及意义摘要【目的】本研究旨在观察RalA(Ras-reletedproteinA)、RhoC(RasholomogyC)mRNA在非小细胞肺癌组织中的量变化,探讨在非小细胞肺癌中RalA、RhoCmRNA的表达与其发生发展及侵袭转移之间的关系。【方法】选取60例经病理学诊断的非小细胞肺癌(Non-smallcelllungcancerNSCLC)患者,利用实时荧光定量PCR分别检测RalA与RhoCmRNA在非小细胞肺癌组织及癌旁组织中的水平,分析RalA、Rho
2、C表达量与非小细胞肺癌各II缶床病理学指标之间的关系。统计学处理分析采用SPSSl1.5统计软件,通过完全随机设计资料方差的统计方法,分析各亚组之间的差别,P3、2-△△c‘的值分别为;0.681±0.245;1.083±0.94P=O.039/:'<0.05),且与淋巴结转移无明显相关∽0.05)。RalAmRNA,RhoCmRNA在鳞癌和腺癌的亚组中表达量均无差异(P4、87909EXPRESSIONANDSIGNⅡ嘎CANCE0FRALAANDRHoCMRNAINNON-SM陵LLCELLLUNGCARCINOM【AAbstraetOb5、ectiveToinvestigatethemRNAexpressionofRalAandRhoCinnon-small-celllungcancer(NSCLC)andtheirclinicalsignificance.Metho凼ThequantitiesofRhocandRataweredetectedbyTaqManprobeb6、asedfluorescencequantitativepolymerasechainreaction(FQ-PCR)in60c遗$esoflungcarcinoma,correspondingpara-cancertissues.ResultsInlungcarcinoma,themeanquanititiesofRhoCmRNAwerehigher△△thanthatincorrespondingpara-cancertissues(2‘QWas:!.237士0.852;0.883i-0.382res7、pectivelyP=0.040).andwascloselyrelatedwithlymphnodemetastases;TNMstageandhistologicalgrade.TheAAmeallquanititiesofIulAmRNAwerehigherincorrespondingpara-cancertissues((2‘c‘was:0.681j=0.245:1.083:t0.94respectively尸=o.039)。andnosignificantassociationWasfound8、bcttwenRatAandtymphnodemetastases(只'o.05).InNSCLC。bothRataandl孙oCmRNAhadnodifferencebetweensquamouscellcarcinomaandadenocarcinoma.(J哆O.05)ConclusionPreservedexpressinofRhoCanddefectiveexpressinofRalAcanbeconcernedwithNSCLC.Postgraduatestudent:ChenDan(Onto9、logy)DirectedbyProf.ZhuangYu[Keywords]Non-smull-celllungcancer;RhoC;RalA;ReaI-TimePCR3引言引言在20世纪中叶,随着经济的发展和吸烟的流行,肺癌的发病率和死亡率迅速上升的现象首先出现在发达国家,发展中国家紧跟其后,根据来自我国全国肿瘤办公室的报告,国内肺癌的发病率和死亡率均占城市恶性肿瘤之首。据WHO统计,全世界肺癌新发病例2002年时在135
3、2-△△c‘的值分别为;0.681±0.245;1.083±0.94P=O.039/:'<0.05),且与淋巴结转移无明显相关∽0.05)。RalAmRNA,RhoCmRNA在鳞癌和腺癌的亚组中表达量均无差异(P4、87909EXPRESSIONANDSIGNⅡ嘎CANCE0FRALAANDRHoCMRNAINNON-SM陵LLCELLLUNGCARCINOM【AAbstraetOb5、ectiveToinvestigatethemRNAexpressionofRalAandRhoCinnon-small-celllungcancer(NSCLC)andtheirclinicalsignificance.Metho凼ThequantitiesofRhocandRataweredetectedbyTaqManprobeb6、asedfluorescencequantitativepolymerasechainreaction(FQ-PCR)in60c遗$esoflungcarcinoma,correspondingpara-cancertissues.ResultsInlungcarcinoma,themeanquanititiesofRhoCmRNAwerehigher△△thanthatincorrespondingpara-cancertissues(2‘QWas:!.237士0.852;0.883i-0.382res7、pectivelyP=0.040).andwascloselyrelatedwithlymphnodemetastases;TNMstageandhistologicalgrade.TheAAmeallquanititiesofIulAmRNAwerehigherincorrespondingpara-cancertissues((2‘c‘was:0.681j=0.245:1.083:t0.94respectively尸=o.039)。andnosignificantassociationWasfound8、bcttwenRatAandtymphnodemetastases(只'o.05).InNSCLC。bothRataandl孙oCmRNAhadnodifferencebetweensquamouscellcarcinomaandadenocarcinoma.(J哆O.05)ConclusionPreservedexpressinofRhoCanddefectiveexpressinofRalAcanbeconcernedwithNSCLC.Postgraduatestudent:ChenDan(Onto9、logy)DirectedbyProf.ZhuangYu[Keywords]Non-smull-celllungcancer;RhoC;RalA;ReaI-TimePCR3引言引言在20世纪中叶,随着经济的发展和吸烟的流行,肺癌的发病率和死亡率迅速上升的现象首先出现在发达国家,发展中国家紧跟其后,根据来自我国全国肿瘤办公室的报告,国内肺癌的发病率和死亡率均占城市恶性肿瘤之首。据WHO统计,全世界肺癌新发病例2002年时在135
4、87909EXPRESSIONANDSIGNⅡ嘎CANCE0FRALAANDRHoCMRNAINNON-SM陵LLCELLLUNGCARCINOM【AAbstraetOb
5、ectiveToinvestigatethemRNAexpressionofRalAandRhoCinnon-small-celllungcancer(NSCLC)andtheirclinicalsignificance.Metho凼ThequantitiesofRhocandRataweredetectedbyTaqManprobeb
6、asedfluorescencequantitativepolymerasechainreaction(FQ-PCR)in60c遗$esoflungcarcinoma,correspondingpara-cancertissues.ResultsInlungcarcinoma,themeanquanititiesofRhoCmRNAwerehigher△△thanthatincorrespondingpara-cancertissues(2‘QWas:!.237士0.852;0.883i-0.382res
7、pectivelyP=0.040).andwascloselyrelatedwithlymphnodemetastases;TNMstageandhistologicalgrade.TheAAmeallquanititiesofIulAmRNAwerehigherincorrespondingpara-cancertissues((2‘c‘was:0.681j=0.245:1.083:t0.94respectively尸=o.039)。andnosignificantassociationWasfound
8、bcttwenRatAandtymphnodemetastases(只'o.05).InNSCLC。bothRataandl孙oCmRNAhadnodifferencebetweensquamouscellcarcinomaandadenocarcinoma.(J哆O.05)ConclusionPreservedexpressinofRhoCanddefectiveexpressinofRalAcanbeconcernedwithNSCLC.Postgraduatestudent:ChenDan(Onto
9、logy)DirectedbyProf.ZhuangYu[Keywords]Non-smull-celllungcancer;RhoC;RalA;ReaI-TimePCR3引言引言在20世纪中叶,随着经济的发展和吸烟的流行,肺癌的发病率和死亡率迅速上升的现象首先出现在发达国家,发展中国家紧跟其后,根据来自我国全国肿瘤办公室的报告,国内肺癌的发病率和死亡率均占城市恶性肿瘤之首。据WHO统计,全世界肺癌新发病例2002年时在135
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