主动脉夹层stanford+某型microrn某的表达与调控机制分析

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1、目录摘要.....................................................IAbstract...............................................III主要英文缩略词表.......................................VII第一章前言..........................................11.1StanfordA型主动脉夹层的病理机制..................11.2非编码RNA............................

2、............11.3miRNA对血管平滑肌细胞的调控.......................21.4miRNA与StanfordA型主动脉夹层....................3第二章研究方法.........................................52.1实验材料..........................................52.1.1实验对象及分组...............................52.1.2样本处理和保存...............................52.1.3

3、临床资料.....................................62.1.4实验试剂、仪器及设备.........................62.2实验方法..........................................62.2.1样本totalRNA的提取及质量检测...............62.2.2totalRNA的去磷酸化及标记反应................82.2.3芯片清洗及扫描...............................82.3实验数据的统计分析.........................

4、.......82.3.1临床数据的分析...............................8V万方数据2.3.2miRNA表达分析................................92.3.3差异表达microRNA的靶基因预测与信号通路分析..9第三章结果.........................................103.1临床资料的比较...................................103.2miRNA芯片检测结果................................103.3miRNA的靶基因预

5、测及信号通路分析..................12第四章讨论.........................................18第五章结论.........................................23第六章参考文献.......................................24第七章文献综述非编码RNA与胸主动脉夹层的研究进展....29研究生期间的科研成果....................................39致谢........................................

6、...........40附图..................................................41VI万方数据主要英文缩略词表缩略词英文全称中文全称ADaorticdissection主动脉夹层ECMextracellularmatrix细胞外基质EDsEhlers-danlossyndrome埃勒斯-当洛综合征FBN1fibrillin-l原纤蛋白-1基因FGFR3fibroblastgrowthfactorreceptor3成纤维母细胞生长因子受体3lncRNAslongnon-codingRNAs长链非编码RNAmRNAmessenger

7、ribonucleicacid信使核糖核酸MAPKmitogen-activatedproteinkinase促分裂原活化蛋白激酶MMPsmatrixmetalloproteinases基质金属蛋白酶miRNAmicroRNA微小核糖核酸MAPKmitogen-activatedproteinkinase丝裂原活化蛋白激酶MFsmarfansyndrome马凡综合征ncRNAsnon-codingRNAs非编码RNAPDGFplateletderivedgrowthfactor血