液相色谱-质谱联用技术在小型家用电器安全和药物代谢方面的应用

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1、摘要Theexperimentusedtoluene：methanol=10：1鹤extractingsolventandheatingbackflow4hours．TheextractsolutionWaSdriedbyN2，dissolvedbytheinitialmobilephase，vortexed，centrifugedandfilmed．AftertheseparationbyBEHClscolumn，thethreeisomeridesofHBCDweredetectedbymassspectrometryinthe

2、negativeelectrosprayionizationwithmultiplereactionmonitoring(MRM)mode．Thethreecompoundswereseparatedeffectivelyin3min．Thelinearrangesofthecompoundswere1．6．32．4肛g·mL’1forHBCDswithcorrelationcoefficientgreaterthan0．996．Thelimitofdetection(LOD)andthelimitofquantitation(LO

3、Q)forHBCDwereO．1and0．5pg·mL～．andtherecoverieswas68％一75．2％．ThismethodWassuccessfullyusedindeterminationtherealsamples．(2)TheexperimentestablishedsimultaneouslydetectionofbisphenolA(BPA)，tetrabromobisphenolA(TBBPA)，andperfluorooctanoicacid(PFOA)insmallhouseholdelectrical

4、appliancesproductsbyacceleratedsolventextraction(ASE)一UPLC-MS／MS．WedesignedL9(34)orthogonaltableforASE，andselectedtheoptimumextractionconditions：extractingsolventwastoluene：methanol==10：1；thepressurewas1500psi；thetemperatureWas100"(2；heatingtimewas5min；staticextraction

5、timeWas20min；circular3times；andN2purgedtimeWaslOOs；thevolumeforwashingWas20％．TheextractsolutionWasdriedbyN2，dissolvedbytheinitialmobilephase，vortexed，centrifugedandfilmed．AftertheseparationbyBEHClscolumn，threecompoundsweredetectedbymassspectrometryinthenegativeV北京化工大学硕

6、上论文electrosprayionizationwithMRMmode．Thethreecompoundswereseparatedeffectivelyin3min．Thelinearrangesofthecompoundswere1-100ng·mL一，0．1-10I．tg·mL～，10．1000ng·mL～forBPA，TBBPA，PFOAwithcorrelationcoefficientgreaterthan0．996．TheLODsforthethreecompoundswere0．1，10，lng。mL～andthe

7、LOQswere0．5，50，5ng·mL～individually．Throughtheextemalstandardmethodtoquantitative，therecoveriesforBPA，TBBPA，PFOAwere84％-92％，76％-82％，72％一74％respectivelywithRSDlowerthan5％．Themethodandtheresultwereconfirmedbyliquidchromatography。iontrap‘timeofflightmassspectrometry(LC—IT．

8、TOF)．TheresultsweregoodandthemethodWassuccessfullyusedindeterminationtherealsamples．2．Establisheddetectionofnaloxone，