甜橙茎陷点型弱毒株基因型的时空分布与其田间防效评估

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1、万方数据AostraetCrossprotectionagainststem··pittingtristezaofsweetorangebytwomildCitrustristezavirusisolatesandthespatialandtemporaldistributionofCTVgenotypesMajor：PlantPathologyMasterCandidate：TaoZhenzhenSupervisor：Prof．ZhouChangyongAssociateProf场AbstractTristezacausedbyCitrustristezavirus(CTV)iso

2、neofthemostimportantdiseasestothecitrusindustry,andhascausedhugeeconomiclossthroughouttheworld．WiththestructureadjustmentofthecitrusvarietiesinChina，thecultivationproportionofpomeloandsweetorangehadbeenpromoted，thedamagecausedbysternpittingisolatesofCTVhasbeenbecomingmotesevere．Bothdomesticando

3、verseasreasearchdatashowedthatmildstraincrossprotection(MSCP)wouldbethemosteffectivemanagementagainststempittinginareaswherevirulentCTVisolatesandToxopteracitricida(Kirkaldy)werecommonlydistributed．DuetothegenotypecompositionofsevereandmildstrainsofCTV,associatedwiththecontroleffectofmildstrain

4、，quantitativedectectionofthedifferentCTVgenotypesofthemildstrainwouldhelpfurtherstudyonthemechanismofMSCP．Inthisstudy,apairofprimersT3-4F／RweredesignedwithintheORFlaofT3genotype，andtheSYBRGreenIreal-timeRT-PCRdetectionsystemwagestablishedwithoptimizedreactioncondition．Andthen．itssensitivityandr

5、eproducibilitywereestimated．ArapidspecificSYBRGreenIRT-PCRmethodfordetectingofT3genotypewasestablished，andusedtomonitorthedifferentgenotypecontentofthemildstrainsCTI1andCT31infield，togetherwithreportedreal-timefluorescencequantimtivemethods，detectingT30，T36，VTgenotypes．Inthemainwhile，theeffecto

6、fMSCPWasevaluedinthefield州tllmildstrainsCTI1andCT31pre-inoculated．Thefollowingresultshavebeenachieved：1．TheSYBRGreenIRT—qPCRdetectionsystemwasestablishedwithoptimizedreactioncondition．Thissystemhadstrongspecificityandhi曲sensitivitytoT3genotypeCTV,whichwas100timeshigherthanthatofconventionalRT-P

7、CR．Agoodlinearcorrelation(ha=0．992)obtainedfromtwoIII万方数据西南大学硕士学位论文standardcurveofcRNA．TheamplificationefficiencyWaS97．1％．Three-timerepeatsrevealedthatthecoefficientsofvariationbetweentheintra-andinter-assaywerebo廿1within2．94％．Itc