β-酪啡肽-7对ⅰ型糖尿病大鼠心肌损伤的干预作用与其机制

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1、ABSTRACTTHEINTERVENTIoNEFFECTANDMECHANISMoFp—CASoMoRPHIN一7ONDIABETICCARDIOT讧YOPATHYEffectofp—Casomo印h证一7onmycordialdamageinSTZ．induceddiabeticratswereexploredThisstudyinthisstudy．Theresearchconsistsofthreeparts：1Establishmentofdiabeticcardiomyopathymodelw

2、ithstreptozotocinToinvestigatetheeffectofstreptozotocinondiabeticcardiomyopathyinrat．(ToapplystreptozotocinforinducingtypeIdiabetes，twenty．fivemaleSDratswererandomlydividedinto2groups，15ratswereselectedtobemodel，otherswereregardascontr01．Themodelgroupwhic

3、hwasinjectedwith60megkgofSTZoncethroughperitonealinjection(allratswerefedbutnowarterbeforeinjectionfor12h)，andcontrolgroupwhichwasinjectedwithcitratebuffersolutionofthesamevolume．Thefastingbloodglucoseweretestedafter24h，72hintakingbloodfromvenacaudalisaft

4、erinjection，wetookfastingbloodglucoseover16·6mmol／Lasmodel．Weraise8ratsforsuccessfulmodelandcontrolgrouprandomlV．Anddailywaterintake，urinaryoutput，foodintake，andbodyweightofratsina11thegroupswereobserved；after30daysoraladministration，wetookbloodfi'omjugul

5、arvein：andexecutethem，conductexperimentusingserum，cardicmusle：①measurebloodglucosebyoxidationenzyme；calculatecardiacmuscleindex；②testinsulinandglucagonbyRIA；③enzymeactivityforNa+K+-ATPandLDH；④testasbiochemicalindicatorforoxidativestressinblood．①Modelgroup

6、showedclinicalmanifestationofdiabetesmellitus(polydipsia，polyphagia，polyuria)，themodelonesmaintainoverl6．61Tinlol／Lwhichshowedsignificantincreaseinthelevelsbloodglucose妒

7、linsen】mp

8、ntr01．Inconclusion，peritonealinjectionSTZcouldinducemodeloftypeIdiabeticcardiomyopathy：themodelonesshowedclinicalmanifestationofdiabetesmellitus(polydipsia，polyphagia，andpolyuria)；hyperglycaemia觚doXldatlvestresswere