含氨基功能基三(22c3-二溴丙基)异氰脲酸酯半抗原的合成

《含氨基功能基三(22c3-二溴丙基)异氰脲酸酯半抗原的合成》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、含氨基功能基三(2,3---溴丙基)异氰脲酸酯半抗原的合成AbstractPersistentOrganicPollutants(POPs)isakindoftoxicorganiccompoundswhichcanexistintheenvironmentforalongtimeandtransportalongdistanceintheenvironment．Theexistenceofthesecompoundsposesadverseeffecttohumanbeingsandecosystem．Recentadvancesin

2、theresearchofartificialantigenhaveshownthatartificialantigenscanbeavaluableapproachforthetreatmentofsomediseasesaswellasthedetectionofpesticideresidues．Bycouplinghaptenstoanappropriatedcarrier，artificialantigencaninduceanimalstoproducehapten-specificantibody．Basedonthisp

3、rincipleanewanalyticalmethod，immunologicalanalysishasbeenrecentlydeveloped．Comparedtotheconventionalanalyticaltechnologies，suchaschromatographicmethods，thismethodoffersanalternativewithhighspecificity'sensitivityfortheanalysisofalargenumberofsamplesinashorttime．Tris(2,3-

4、dibromopropyl)isocyanurate(TBC)isaheterocyclichexabrominatedadditiveflameretardantthatpossessthetypicalcharacteristicsofpersistentorganicpollutants(POPs)，whichisresistanttophotodegradation，durable，andthermallystableandhasadverseeffecttotheecosystemsandhumanhealth．TBCisas

5、mallorganiccompoundwhichisnon—immunogenic．Hence，itisnecessarytodesignappropriatechemicalstructuresthatcanbecovalentlycoupledtoacarrierprotein．Inordertofindthehaptenstructuresandactivityrelationshipofthefinalantibody,wearecommittedtothefollowingtworesearches：Firstly，inter

6、mediatecompound3wassynthesizedviaIntra-rearrangementreactionandtheyiledwasoptimizedto69％，thenthefirstseriesofhaptenswitharomaticfunctiongroupforTBChavebeensuccessfullyobtainedviaWilliamensonetherreaction，Nucleophilicsubstitutionreaction，Bromination，Gabrielreaction，Reduct

7、ivereaction．Thereasonforintroducingaminetothestructureisthatitcouldreactwithcarboxylicgroupoftheproteincarriertoformpeptidebond．WedesignedfourhaptensforTBCwithdifferentlengthofalkylchainsinordertofindhowthelengthofspacerarmsinfluencetheconjugationofgeneratingantigensandt

8、hefinalproductionofantibody．Secondly,theotherserieshaptenswithaliphaticfunctiongroupforTBChavebeensucce