我国伤寒和甲型副伤寒沙门菌pfge的分型分析

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1、我国伤寒和甲型副伤寒沙门菌PFGE的分型研究英文摘要4．O)softwarepackagesandthenthepatternswereclusteredbyUnweighedPairGroupMethod(UPGMA)usingArithmeticaverage．Theclusteringsimilaritycoefficientwasbetween41．4％and100％．Wefinallygot456differentXbaIpatterns．Eachpatternincluded1to44strainsand2strains

2、onaverage．38kindsofpa：ttemswere‘discoveredindifferentprovincesandtheother418pattemsbelongedtotheuniquepat￡emofeachprovince．Therewere63kindsofpatternsdiscoveredindifferentyearsandtheother393patternsbelongedtotheuniquepa：ttemofeveryyear．Numbersofcloneswithsignificantdiff

3、erencewereexistedindifferentareasandappearedatdifferenttime．Theseresultssuggestedthat＆typhistrainsisolatedinChinahadalargevariability．However,thesamepaRemdiscoveredinvariousyearsindicatedthat&typhistrainswererelativelystableincontinualvariation．Thisdatabasehaspotential

4、notonlytoconnectandsharedatawithinternationallaboratorysurveillancenet,butalsotoanalyzeChinatyphoidfeversurveillanceandtoserveforinternationaltyphoidfeverprewamingandemergencyreaction．TheincidencerateofS．paratyphiAWaslow,between0．4per100thousandand3．7per100thousand，and

5、mostcasesweresporadicinthedevelopedcountries．SalmonellaPFGEstandardizationprotocolreleasedbyPulseNetistargetedmainlyatSerotype＆typhimurium．Inrecentyears，¶typhiAcausedepidemicinChinaandSoutheastAsiacounties．Duringthesurveillanceandannalysisof¶typhiAisolates，wefo

6、undthatthediscriminationof＆typhimuriumPFGEtypingmehodWaslimitedwhenitappliedtotheanalysisofS．paratyphiAstrains．InordertoexplorethesuitablePFGEanalysisprotocolforSparatyphiAstrains，wescreenedandoptimizedtheDNAendonucleaseandPFGEelectrophoresisparameters，andchosethreekin

7、dsofrestrictionenzymeXbaI，印口IandXhoIaspreferredendonuclease．AmongseveralelectrophoresisparametersofS．paratyphiAPFGEmoleculartyping，weselectedthePFGEanalysisprotocolofapreferredenzymebycomparingtheelectrophoresisparametersofeachenzyme，whichhadthebestdiscriminatoryabilit

8、y．Wechosedifferenteleetrophoresisparametersforeachenzymeontheanalysisof．S：paratyphiAstrains．Bycomparingthediscriminat