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ID:32074081
大小:2.10 MB
页数:47页
时间:2019-01-31
《体外测定生物材料诱导成骨活性方法的建立和应用》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、ESTABLISHMENTANDAPPLICATl0NOFANEWASSAYINVITROOFOSTEOINDUCTIVITYOFBIOMATER工ALSPostgraduateJIANGChuan-jieTutorQIJian-hongProfessorLUShi—biAcademicianSpecialitySurgeryABSTRACTLargebonedefectsandspinefusions,bonetumors,reconstructivesurgeryneedmassivebonegraftmaterials,butautogene
2、iebonegraftcan’tprovideelloughbonegraftsManyosteoconductiveandosteoinductivebonegraftsubstitutivematerialshadbeenusedinorthopedics.Thebioactivityofsomeosteoinductivebonegraftmaterialssuchasdemineralizedbonemarix,compositedmaterialscontainingnativebonemorphogeneticprotein,andot
3、herbioactivematerialscontainingrecombinedgrowthfactorsdependsontheactivityofgrowthfactorsinit,butthebioactivityofthesematerialsiseasytObeinactivatedbysometreatments,suchaspreparation,storage,sterilizationandSOon.Itisnecessarytoevaluateosteoinductivityofthesebioactivitematerial
4、sfortheeffectiveuseofbioactivematerials.Thetraditionalassaymethodinanimalbodyforevaluatingbioaetivityofmaterialshavemanyshortcomings,suchassacrificinganimals,longtermofassay,manyinterferencefactorstotestresult,standardizingandsequencingdifficultly,highcost,andSOon.ItCan’tsatis
5、fytheactualneed.Thisresearchwillestablishanewstandardizedassaymethodinvitroforevaluatingosteoinductivityofbioactivematerials.Thisassayshouldbeconvenient,repetitive,standardandlowcost,andCanreplacethetraditionalassaymethodinvivo.Thisresearchwillalsoattempttoassayosteoinductivit
6、yofkindsofbioactivematerialsandcompare、Ⅳimeachother.Objective:Toestablishanewstandardizedassaymethodinvitroforevaluatingosteoinductivityofbioactivematerials,whichisconvenient,repetitiveandlowcost,canreplacethetraditionalassaymethodinvivo.Besides,thisresearchwillattempttoassayo
7、steoinductivityofkindsofbioactivematerialsandcomparewitheachother.3Methods:Partone:EstablishmentofanewassayinvitroforevaluatingosteoinductivityofbioactivematerialsThegoalDBMwaspreparedusingfreshcorticalbonebyaserialofbiologicalandchemicaltreatments.BMP·2inthegoaldemineralizedb
8、onematrix(DBM)wasdeterminedbyimmunohistomicaltestGoalDBM(grou
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