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ID:32073283
大小:1.35 MB
页数:54页
时间:2019-01-31
《水产品中副溶血性弧菌的elisa快速检测》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、上海水产大学硕士论文PAbto坳islxl04cfu/mL;ThecrossingtestandinhibitiontestshowsthatthePAbisstronglyspecial.Sothisworkwilllayafoundationforlaterwork.2EstablishmentofIndirectELISARapidMethodInthispaper,theoptimumreactionconditionofeverydetectionprocedurewasdeterminedt
2、hroughfiltrationexperiment,andtheoptimumworkconcentrationoftheAgandantibodybyusingcheckerboardtitration.Afterthemethodwasstandardized,thedetectionparameterswerebelow:Usingthenewcoatingmethod(dryingcoatingat60℃1;ThecoatingconcentrationofAgis107cfu/ml;Clos
3、ingtimeis2.5h;Theworkdilutionoffirstantibodyis1:4000;TheincubationtimeofAgreactingwithfirstantibodyis75min;Thedilutionofthesecondaryantibodylabeledenzymeisl:1000;Theincubationtimeoffirstantibodyreactingwiththesecondaryantibodylabeledenzymeis60min;Thereac
4、tiontemperatureofenzymeandsubstrateis30"C,timeis15min;theincubationtemperatureis3TC;UsingthemethodtodetectthebacteriumsuspensionofEp,thedetectionlimitandtimeare104cfu/mland8hseparately.3IndirectELISARapidMethodforDetecting印TheindirectELISArapidmethodford
5、etecting印WasestablishedbyusingtheantibodyastheIn'stantibody,andthegoatanti—rabbitlgG-HRP髂thesecondaryantibodylabeledenzymeseparately.ThenthestandardizedmethodWasusedfordetectingtest.ThecrossingtestresultshowsthatthecrossreactionbetweenthePAbandotherbacte
6、riaisnegative.TheinhibitiontestshowsthatthePAbisspecialstrongly,andtheinhibitionratioreaches86.53%.Usingthismethodtodetectseafoodinfectedwithbacteriaartificiallyandactualseafoodrespectively,thelowestdetectionlimitis104cfu/ml,thedetectiontimeis8h;However,
7、thedetectionlowerlimitWasincreasedto10’cfu/mlbyculturingthebacteria.Thismethodisconsistentwiththeconventionalmethodtestresultcompletely.Therefore,itCallofferanexact,rapidmethodfordetecting脚inseafood,andalsohasveryhighpracticalapplicationValRe.4Developmen
8、tofindirectELISAdiagnostickitforrapiddetecting吻inseafoodThecomponents,userintroduction,operationnoticesofindirectELISAdiagnostickitforrapiddetecting印inseafoodweregiven.Thenwecarryouttheseexperimentaboutstoragelifeofthekit’
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