山苍子油对皮肤癣菌的药物敏感性实验与临床实验

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1、英文摘要ThedrugsensitivitytestofDermatophytetoLitseacubebaoilandclinicalexperimentABSTRACTObjective:clinically,superficialmycosisisakindofcommonandfrequently-occurringdiseasewhichisinfectedbyDermatophyte.Itisusuallytreatedwithantifungaldrug.Howeverthedrugresistanceoffungiiscontinuouslyari

2、singandthehi沙relapseratepuzzlesufferersandmedicalworkers.Itimpelsscientificresearchworkerscontinuouslytolookforthenewbroadspectrum,highperformanceandcheapantifungaldrugs.Inthepastfewyears，TheantifungaldrugofthetraditionalChinesemedicineweremadegreatprogress,andmanyantifungaldrugofthet

3、raditionalChinesemedicinewereselectedout,ofwhichLitseacubebaoilisonekind.ThistestwasconsultedUSANationalCommitteeforClinicalLaboratoryStandards(NCCLS)RecommendMethodforbrothdilutionantifungalsusceptibilitytestingofyeasttodeterminetheminimuminhibitoryconcentration(MIC)ofLiseacubeabaoil

4、aboutfourkindsofDermatophyte.WeobservedtheultrastructuralchangesofTrichophytonmentagrophytesbeforeandaftertestthroughelectronmicroscope.Moreover,wemadeLitseacubebaoilbecream,andweusedthecreamtotreattineaoffeetandhands,observingcurativeeffect.Weusedgrinderstogrindhyphaeandcountedthemby

5、bloodcellcountingplatein一一.一一一一.一一.一4英文摘要ourtest.Sowemadesureofconcentrationofthefungisuspensionandresolvedatoughproblemthattheamounttohaveaninoculationisdifficulttocontrol.Allhadmadetheresultsbescientificandbelievable.Methods:WeseparatedTrichophytonrubrumEpidermophytonfloccosumTricho

6、phytonmentagrophytesMicrosporumcanisfromthelesions.Identifythemoncemore.ThentheywereculturedinSabouraud}sdextroseagar(SDA),cultured10daysat270C.Atlast,webroughtouthyphaeandgrindedthemWecountedhyphaethroughbloodcellcountingplateandadjustedtheconcentrationoffungisuspensionbetween1XlObcf

7、u/mland5X106cfu/ml.LitseacubebaoilwasdilutedbyRPMI-1640liquidmediumtobeconcentrationfrom80000ug/mlto156ug/ml.FluconazolwasdilutedbyRPMI-1640liquidmediumtobeconcentrationfrom128ug/mlto0.25ug/mlGet100u1medicineliquidtotheholeof1一10fileof96holeplateinturn.Everyconcentrationmedicineliquid

8、wasad