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ID:32069775
大小:1.93 MB
页数:65页
时间:2019-01-31
《人源性饲养层制备的标准化操作》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、硕士学位论文中文摘要克隆的培养,并在过去的一年半时间内保持hESCs克隆的的分化率和正常核型。关键词人胚胎干细胞,人成纤维细胞,饲养层细胞,人源性IV硕士学位论文英文摘要Peopleareattraetedbyhumanembryonicstemcells(bESts)sincetheirdiscovering,astheyhavethecha“K:缸:嚣ofself-renewingandploripotentabilities.AnykindofhESCsresearchisbasedOnthep
2、remisothatwecangethESCssteelyinalongterm.Co-culturewithmouseembryonicfibroblastFeeder(mousoFeeder)istheearliestwaytogethESCs,immediatelyafterthathumanembryonicfibroblastFeederfaumanFeeder)takesintouse,scientistsculturehESCsSOlongtimethatthestabilityofth
3、esetwomethodscanbewellapproved.However,themostwidelyusedFeederwhichismadebymolkqeembryonicfibroblastsdonotcommitthedemandingsofclinic,toaccomplishthegoalsofhESCs’clinicaltransplantweshoulddoourbesttoavoidtheuseofanyanimalmaterials,especially,allimalcell
4、s.Intheseyears,somelabsfoundthatthereisanotherwaytoculturehESCsbesidesoo--culturewithothercells,itiscalledFeederfreeculturesystem,butitisstirnotsu糟aboutthatwhetherthiswaycankeephESCsstablewithnormalkaryotypes.Forthesereasopi,co-culturewithFeederwhichism
5、adebyhumanembryonicfibroblastscomestobeOurbestchoicerightnow.IfwewantgetlargequantifiesofundifferentiatedhESCs,weshouldfirstlygetenoughhumanembryomcfibroblastsandasteadyhumanFeeder-makingprotoc01.We’vedonesomeexperimentstosolvetheproblemswe’veeverfoundi
6、nOUI"humanFeedermaking,andthenformulatedanewstandardFeeder-makingprotoc01.Aims:1.CollectenoughhumanembryonicfibroblastsforFeeder-making;2.Dosomeexperimentstosolvetheproblemswe’veeverfoundinOurhumanFeedermaking;V硕士学位论文英文摘要3.FormulateanewstandardFeeder-ma
7、kingprotocolaccordingtothedatawe’Vegotfromtheseexperiments.flOthatWe’11haveaguidelinewhenWelifemltldnghumanFeeder.1VInterkqlsandmethodslWechoosethewholefetalbodythatabandonedinabortionsurgeryfromChangshaMaternalAndChildCareServicetodoOUI"collectionofhEF
8、s.ThefirststepofcollectionWS.StOseparateskinandmusclefromthelimbsoffetalbodymeehanismly,then,hEFsWaSderivedthroughorganizationculturing,andpasagedbyT/E.Wedotheexaminations011eachb_EFsstrainthatcomesfromdifferentfetalbodies:Chlamy
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