人源性饲养层制备的标准化操作

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1、硕士学位论文中文摘要克隆的培养，并在过去的一年半时间内保持hESCs克隆的的分化率和正常核型。关键词人胚胎干细胞，人成纤维细胞，饲养层细胞，人源性IV硕士学位论文英文摘要Peopleareattraetedbyhumanembryonicstemcells(bESts)sincetheirdiscovering,astheyhavethecha“K：缸：嚣ofself-renewingandploripotentabilities．AnykindofhESCsresearchisbasedOnthep

2、remisothatwecangethESCssteelyinalongterm．Co-culturewithmouseembryonicfibroblastFeeder(mousoFeeder)istheearliestwaytogethESCs,immediatelyafterthathumanembryonicfibroblastFeederfaumanFeeder)takesintouse，scientistsculturehESCsSOlongtimethatthestabilityofth

3、esetwomethodscanbewellapproved．However,themostwidelyusedFeederwhichismadebymolkqeembryonicfibroblastsdonotcommitthedemandingsofclinic，toaccomplishthegoalsofhESCs’clinicaltransplantweshoulddoourbesttoavoidtheuseofanyanimalmaterials，especially，allimalcell

4、s．Intheseyears，somelabsfoundthatthereisanotherwaytoculturehESCsbesidesoo--culturewithothercells，itiscalledFeederfreeculturesystem,butitisstirnotsu糟aboutthatwhetherthiswaycankeephESCsstablewithnormalkaryotypes．Forthesereasopi，co-culturewithFeederwhichism

5、adebyhumanembryonicfibroblastscomestobeOurbestchoicerightnow．IfwewantgetlargequantifiesofundifferentiatedhESCs，weshouldfirstlygetenoughhumanembryomcfibroblastsandasteadyhumanFeeder-makingprotoc01．We’vedonesomeexperimentstosolvetheproblemswe’veeverfoundi

6、nOUI"humanFeedermaking,andthenformulatedanewstandardFeeder-makingprotoc01．Aims：1．CollectenoughhumanembryonicfibroblastsforFeeder-making；2．Dosomeexperimentstosolvetheproblemswe’veeverfoundinOurhumanFeedermaking；V硕士学位论文英文摘要3．FormulateanewstandardFeeder-ma

7、kingprotocolaccordingtothedatawe’Vegotfromtheseexperiments．flOthatWe’11haveaguidelinewhenWelifemltldnghumanFeeder．1VInterkqlsandmethodslWechoosethewholefetalbodythatabandonedinabortionsurgeryfromChangshaMaternalAndChildCareServicetodoOUI"collectionofhEF

8、s．ThefirststepofcollectionWS．StOseparateskinandmusclefromthelimbsoffetalbodymeehanismly,then，hEFsWaSderivedthroughorganizationculturing,andpasagedbyT／E．Wedotheexaminations011eachb_EFsstrainthatcomesfromdifferentfetalbodies：Chlamy