【硕士论文】脂代谢紊乱对大鼠肾小管上皮细胞rOAT3基因表达的影响.pdf

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1、脂代谢紊乱对大鼠肾小管上皮细胞rOAT3基因表达的影响摘要目的研究高脂血症对大鼠肾小管上皮细胞rOAT3(ratorganicanion％ransporter3)基因表达的影响，探讨脂代谢紊乱与痛风及高尿酸血症的相关性。方法以雄性OLETF大鼠为研究对象，以雄性LETO大鼠(与OLETF大鼠同系)为对照。基础饲辎饲养至7周龄，剪尾取血保存，1l周龄时所有大鼠用水合氯醛腹腔注射麻醉，左肾静脉取血，活体取肾皮质液氮保存，采用生化仪检测Jf【L脂、尿酸、血糖、尿素氮、肌酐，采用逆转录一聚合酶链反应(RT—PCR)方法检测rOAT3活性和mRNA水平。大鼠生化指标和rOAT3表达水平用(X

2、±S)表示，组间两两比较选用t检验。结果(1)5周龄OLETF大鼠的血糖、血尿酸、血脂水平与对照组LETO大鼠相比无明显差异：(2)7周龄OLETF大鼠血脂(TG、．TC)水平比对照组LETO大鼠增高但差异无显著性；(3)1l周龄OLETF大鼠的血脂水平明显高于对照组(P

3、的发病相关。关键词：脂代谢紊乱：OLETF大鼠；rOAT3；基因表达TheinfluenceofdislipidemiaonrOAT3inrenaltubularepitheliaofratsABSTRACTObjective：ToinvestigatetheinfluenceofhyperlipidemiaonrOAT3(ratorganicaniontransporter3)inrenaltubularepitheliaofrats．Tomakecleartherelationshipbetweendislipidemiaandthedevelopmentofgoutandhy

4、peruricemiabyanimalexperiments．Methods：Experimentalgroupincludedtenfour—weeks-oldmaleOLETFrats，controlgroupincludedtenfour-week—oldmaleLETOratswhichbelongedtothesamekindwithOI，ETFrats．Theywereallgivenadaptivetreatmentforsevenweeks，duringthetimetheyweretakenoutbloodfromthetailseveryweek．Reserve

5、theserumseperatedfromtheblood．Treateleven-week·oldratswithchloralhydratebyrejectioninabdominalcavity，takebloodfromtheleftkidneyvein，andtakeoutsomekidneycortex．．Theconcentrationofserumlipid、uricacidandglucoseweredeterminedbybiochemistrymethod．TheactivityofrOAT3andthelevelofitsmRNAweredetermined

6、byR％PCR．ThedifferenceofthelevelswasanalyzedbyTtest．Results：(1)Thelewasnostatisticdifferenceinthelevelsofglucose、uricacidandlipidoffive—week—oldratsbetweenthetwogroups．(2)ThelevelsofTGandTCinseven-week—oldOLETFratswerehigherthan，thoseincontrolgroup，buttherewasilostatisticssignificance．(3)Thelev

7、elsofthelipidineleven-week—oldOLETFratswerehigherthanthoseincontrolgroup(P