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1、ABSTRACTCLE(CLAVATA3/Embryosurroundingregion-related)polypeptidehormoneistheplantpolypeptidehormonethatwecaremostinrecenttenyearsandthelargestplantpeptidefamilysofar,involvinginsignaltransductionbetweencellsbycodingagroupofsmallsecretorypeptides,soasto
2、controlthebalancebetweenstemcellproliferationanddifferentiationduringtheplantgrowthanddevelopmentprocess.Italsoplaysakeyroleinregulatingthedevelopmentofplanttissues,especiallyinshootapicalmeristem(SAM),rootapicalmeristem(RAM)andprocambium.Despitetheexi
3、stingresearchofCLEgenefamilyinseveralmodelplants,likeArabidopsisthalianaandOryzasativa,littleisknownaboutCLEpolypeptidegenefamilyinthemodelplantofxylophytalikepoplarandthemodelplantofsarcocarpplantliketomato.Withthemodelplants,poplarandtomato,astherese
4、archobject,thisstudyusesallofthe32CLEgenesofArabidopsisthalianaforgeneretrievalandmininginthephytozomev10.1database,getting50poplarCLEcandidategenes(PtCLE)and15tomatoCLEcandidategenes(SlCLE)intheend.Thenusingbioinformaticsmethods,theCLEgenesofpoplarand
5、tomatoareconductedwithphysicalandchemicalpropertiesanalysis,predictionofsignalpeptide,genestructureanalysis,chromosomelocation,multiplesequencealignment,theconstructionofphylogenetictreebasedontheproteinsequenceandCLE12peptide,thesearchoftheupstreamreg
6、ulationareacis-actingelement,aswellasthedrawingofexpressionheatmap,etc.Inthepoplar,itpicksoutthreepoplarCLEgenesbeinghomologouswithAtCLV3andsixpoplarCLEgenesbeinghomologouswithAtCLE41/44toconstructtheexpressionvectorsfortheninePtCLEgenes,verifyingtheex
7、pressionofitstissuespecificitywiththeuseofreal-timequantitativePCRtechnique.Astothetomato,itverifiestheexpressionofthetissuespecificityofallthe15tomatoCLEgenes,andpicksoutthreespecificexpressiongenes:SlCLE10,12and13.AfterthesyntheticoftheCLE12peptideof
8、theabovethreeSlCLEgenes,thendealwithtomatoseedlingsbyaddingthepolypeptideinvitromethod,itfindsthatthethree12-peptideareabletoinhibittheelongationoftomatomainroot,andSlCLE12hasthemostsignificantinhibitoryeffect,alsothereisplentyofanthocy
