利用组织芯片技术-研究cox-2在胃癌中的表达与其与血管生成的关系

《利用组织芯片技术-研究cox-2在胃癌中的表达与其与血管生成的关系》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、(．．苎塞笙董塑要．．)StudyoftheexpressionofCOX——2ingastriccanceranditsrelationshipwithangiogenesisbyTissueMicroarrayIntroductionGastriccancerisoneofthemalignanttumors，theincidenceandmortalityareincreasing．，111eunlimitedgrowthandmetastasisoftumorcellsisthemostimportantfeature．Itisthemajorreasonforgastric

2、cancerpatients’failuretobecuresanddead．negrowthandmetastasisoftumorsareregulatedbymanyfac-tors．Tissuemicoarray／tissuechipisanewlydevelopedarray—baseshighthroughputtechniquewithaconspicuouscharacteristicofsmallvolumerichininformationthatfacilitatesgeneexpressioninvestigationofverylargenumbersoft

3、umorssimultaneously．Itissuitabletoimmunohistochemistry，DNAorRNAinsitehybridizationwithhishefficiencyandflexibility，anditsresultsarehighlyu-niformwithremarkableCredibilityandcomparability．Manyreportshavedemon-stratedthattissuemicroarray／tissuechipisanewpowerfultechniqueinpatholo-gyoftumorsinvest

4、igation．COX一2isakeyenzymeinPGbiosynthesis．Recently，allenhancedex-pressionofCOX一2hasbeenfoundincasesofcoloncanerandothertumors．andNSAIDscanreducetheriskofcoloncancerinanimalmodels．Manyre．searchsuggestedthatthetulnorgrowthperiodsincludetheangiogenesisperiod．COX-2hastightrelationshipwithgastriccar

5、cinogenesis，buttheactualmecha—nismisnotknown．ObjectiveToexploretheexpressionandclinicalpathologicalsignificanceofCOX一2andMVDimmunohistochemicdllyoncarcinogenesisofgastriccancerbytissue·3·microarraytechnique．Andinvestigatetheirfunctionsininvasionandtherela．tionshipbetweenbiologicalbehaviorsandpr

6、ognosis，expectedtofindmolecularbiologicalmarkerBinpredictingthemetastastiepotentialofgastriccan／2er．Weconstructedtwotissuemicorarrayscontaining225casesofsmall1一咖cylindricalsamplesacquiredfrom104casesofparaffin—embeddedblocksofgastriccancerandnormalgastricmucouswhichderivedfromLiaoningprovinceTu

7、morHospitalandNo．1HospitalofChinaMedicalUniversity。includinggas．triccancerandprecancerouslesionsandnormalgastricmucosa．Detectexpres-sionsofCOX一2andMVDinthetissuemicroarraysofgastriccancerwithEnvi．sion，Theintratumoralmiemvesseldens