胃癌组织中stathmin表达及其与血管生成的关系

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1、摘要目的探讨Stathmin蛋白及mRNA在胃癌发展过程中的意义及其对血管生成的影响。方法1．使用免疫组织化学法检测120例胃癌组织、癌旁组织及30例非肿瘤组织胃黏膜中Stathmin和CDl05的表达，并分析其与胃癌临床病理参数之间的关系，用CDl05标记胃癌组织的微血管并测其密度(MVD)。2．使用实时荧光定量RT—PCR法检测120例胃癌组织中Stathmin及CDl05mRNA的表达情况。分析StathminmRNA表达与临床病理学参数之间的关系，并对Stathmin和CDl05进行相关性分

2、析。结果i．StathminmRNA和蛋白在胃癌组织中的表达。Stathmin蛋白主要表达于肿瘤细胞胞浆。胃癌组织StathminmRNA和蛋白的表达明显高于癌旁组织、非肿瘤组织胃黏膜(F=32．82：X2=59．37，P

3、

4、tathmin；CDl05；血管生成；微血管密度；免疫组化；实时荧光定量RT-PCR法AbstractObjectiveToinvestigatetheroleofStathminproteinandmRNAindevelopmentofgastriccarcinomaanditseffectontumorangiogenesis．Methods1。TheexpressionlevelsofStathminproteinandCD105inpathologicalspecimensfrom120ca

5、sesofgastriccarcinomatissue、adjacenttissuesand30casesofnon-tumortissueswereexaminedbyimmunohistochemistry,TherelationshipbetweenStathminexpressionandclinicopathologicalparametersinhumangastriccarcinomaWasanalysed，andthenCD105markedmicrovesseldensity(MV

6、D)WascountedinordertoanalysetherelationshipbetweenStathminexpressionandMVD．2．Usingreal—timefluorescentquantitativeRT-PCRassayfordetectionofStathminandCD105mRNAexpressin120casesofhumangastriccarcinomatissues．TherelationshipbetweenStathminmRNAandclinicop

7、athologicalparametersinhumangastriccarcinomaWasanalysed，，andthenanalysethecorrelationbetweenStathminandCD105expression．Results1．TheexpressionsofStathminmRNAandproteininhumangastriccarcinomatissues．StathminproteinWasprimarilylocalizedinthecytoplasmofthe

8、carcinomacells．Thepositiverateof．Stathminingastriccarcinomawassignificantlyhigherthanthatinadjacenttissuesorinnon-tumortissues(F=32．82；X2=59．37，P<0．05)2．TherelationshipbetweenStathminexpressionandclinicopathologicalparametersinhumangast