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ID:31986591
大小:3.16 MB
页数:73页
时间:2019-01-30
《甲氨蝶呤红细胞载体的制备与在大鼠体内药代动力学.研究》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、couldenterintothecells.Wealsoreviewedthebrittlenessandchangeofthecellshape.3.TooptimizetheconditionforMTX—RBCperparation,wecalculatedandcomparedtheenvelopmentefficiencyofMTX—RBCwhichmeanstheproportionofMTXcontentincellstothetotalcontentunderdifferent
2、conditionslistedinb(34)orthogonalform,inordertoseekthebestarrangementindifferentconcentrationsofMTX,theratioofhypertonicglucoseandblood,timeofdehydrateandMTXsoakage.4.TheCrOSS-OVerdesignwasusedtoexplorethepharmacokineticsofvenousinjectionofMTX—RBCinr
3、atsandfinditspotentialclinicaluse.WestudypharmacokineticsinratsaftersingleivdoseofMTX—RBCandfreeMTX,bloodsampleswerecollectedat0,5,10,30,60,120,180,240,360,480,600,720and1440minutesafterdifferenttypesadministrationthendetermineddrugconcentrationinrat
4、plasmabyHPLC,calculatedthepharmcokineticparametersbyDASver1.0sofewaretofindoutwhetherithassustainedreleaseeffect.5.ToinvestigatethepreparationofMTX-RBCandfreeMTXandobserveitstissuedistributioninratsafter30,60,120,180,240,300rainadministration.Determi
5、neddrugconcentrationinheart,liver,spleen,lung,kidney,stomachandintestinetoseekwhetherMTX-RBCisadrug-targetingpreparationandCanarrivetocertaintissues.RESULTS:1.Thesamplewasquantifiedovertheconcentrationrangeof0.05.5.00/zg·ml。(r=O.9993).Theminimaldetec
6、tableconcentrationWas20rig·ml~.Inter-dayRSDwere2%·7%andintra-dayRSDwere4%-10%respectively.Thismethodiseasy,sensitiveandaccuratefordeterminationofMTXconcentrationinplasmaofbothhumanandrats.2.TheenvelopmentefficiencytestresultsshowedthatthehigIlestenve
7、lopmentefficiencyineachlevelappearedthatthebestarrangement:theratioofwholebloodto50%glucosesolutionis3:2,thedehydrationtimeis40min,theMTXsolutionconcentrationis250pg。ml一.andthere·enclosedtimeis30min,whichreach41.58%inhumanand54.69%inrats.3.Theosmolal
8、ityonfragilityofcarriererythrocytedidnotchangemuchduringthe.V.ABSTRA(jrpreparationthatcanbeshowedinthecurveofosmolalityonfragility.Normalerythroeytehastheshapeofbiconcavelenswithacentralareaofpallorundermicrograph.Thedehydrated—RBCissmallerinsizeandd
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