基于果蝇rna干扰系-研究parpl基因运动抗心脏衰老功能

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1、组比，5w-Parp1．RNAi．exercise组射血分数升高(P<0．05)。3．3．4果蝇心脏纤维性震颤检测结果随着年龄的增加，果蝇心脏的纤维性震颤指数升高(P

2、默后可导致果蝇生命周期延长。同时，在此运动强度下，l周龄运动干预可以延长果蝇的平均寿命，而5周龄运动干预反而使果蝇平均寿命缩短。4．2l周龄的运动干预能提高果蝇的运动能力，而5周龄的运动干预反而使果蝇运动能力降低，推测此时的运动强度使老龄果蝇的机体产生不适应性，从而影响运动能力。而Parpl基因低表达可以缓解晚期运动干预所致的运动能力降低的趋势。4．3运动干预能提高果蝇心脏的心脏收缩力，从而有助于增强果蝇心脏射血功能。4．4随着年龄的增加，心脏功能逐渐减弱，Parpl基因沉默能明显改善心脏节律不齐指数，从而改善心脏节律。关键词：衰老；运动训练；心脏

3、功能；PARPl基因功能；攀爬能力ⅡIBasedontheRNAinterferencesystemofDrosophilastudiesObjectiveParplsportanti—agingofheartfunctionAbstractUsingdrosophilaasamodelanimalforstudythecardiacaging，andbyinversegravityclimbingindexestablishedmovementanti—agingDrosophilamodel，throughtheUAS-GaL4binarytr

4、ansgenicsystemsandmovementoftheDrosophilageneregulationbyDrosophilacardiacfunctiontestingsystemandbiochemicalmarkersofaging’relatedtesting，tofurtherexplorethefunctionalcandidategenePARP-1sportanti。agingsystemoftheheart,thestudyprovidesevidenceforfunctionalsports—relatedanti‘ag

5、inggene．MetairieandmethodsDrosophila31397strains(buyfromBloomingtonDrosophilaStockCenter)，arm．Gal4andwild．typeW1118strains(HeartDevelopmentResearchCentreofHunanNormalUniversity)，thecollectionexpandedcultivationwithin8—12hemergenceofmalefruitflies2400，andwererandomlydividedinto

6、fourgroups：1weekoldquietcontrolgroup(youngergroup)，1weekexercisetraininggroup(youngerexercisegroup)，5-week．oldsedentarycontrolgroup(agedcontrolgroup)，5-week。oldmovementtraininggroup(youngerexercisegroup)，600／group，20／tube，Fliesarehousedina25。Cincubatorwith50％humidityanda12-hou

7、rlight／darkcycle．Motorcultivatecottonplugbottomofthetubetothelowerareareservedfor8cmasDrosophilasport,exercisefrequencybythemovementofthedevice：theuseofplatformmotion(Drosophilamotiondeviceindependentplatformdevelopedbytheresearchgroup，hasobtainednationalinventionpatents，paten

8、tNo．2010349810．4)speedcontrol，exercisefrequencyis24rev／sec，fl