黄芩苷对低氧肺心病大鼠的保护作用与对p38mapk的影响

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1、万方数据温州医科大学硕士学位论文关键词：慢性肺源性心脏病；黄芩苷；磷酸化的P38丝裂原激活蛋白激酶；基质金属蛋白酶一9TheProtectiveEfiectofBaicalinonCPHDinRatsandEfiectofP38M巳心KAbstractObjective：1．TostudytheeffectofbaicalinonP38MAPKpathwayandMMP-9inhypoxia-inducedchronicpulmonaryheartdiseaseinrats．2．Tostudytheprotectiveeffectofbaical

2、inonhypoxia-inducedchronicpulmonaryheartdiseaseinrats．Methods：24Sprague-Dawleyratswererandomlydividedintothreegroups，thereare8ratsineachgroup．GroupA，hypoxiafor4weeks；GroupB，hypoxia+baicalinfor4weeks；GroupC，normalgroup．Theindexofhemod，7namicsincludingthemeanpulmonaryarterypres

3、sure(maAa)andmeancarotidarterialpressure(mCAP)weremeasured．Wealsodetectedtherightventricle／leftventricle+septum(RV／LV+S)．HEstainingwasdonetodetectthepercentageofpulmonaryarterioleswallarealtotalarea(vesselwellarea

4、totalarea，WA

5、TA)tOreflecttheextentofpulmonaryvascularremodelin

6、g．Besides，thechangesofphospho-P38MAPKandMMP-9proteinsinarterioleswallweredetectedbymeasuringaverageabsorbance(1ightdencity，LD)throughImmunohistochemistrytechnology．Insituhybridization(ISH)wasdonetodetectthechangeofMMP一9mRNAbysimilarmethod．And，atlast，Western··blotttechnologywa

7、susedtodetectthechangeofphospho·-P38MAPKinlung．Results：1．Inhypoxicconditions，themPAP，theRV／畔S)andWA／TAwassignificantlyhigherthanthegroupC(P<0．o1)，andtheinterventionofbaicalincansignificantlyreducemPAP，WA／TA(P

8、cconditions，theexpressionofphosphorylatedP38MAPK，MMP-9inarterioleswallwassignificantlyhigherthanthenormalgroup(P<0．01)；Aftertheinterventionofbaicalin,theexpressionofphosphorylatedP38MAPK，MMP-9waslowerthanthatinhypoxiagroup(P<0．05)．5万方数据温州医科大学硕士学位论文3．Insituhybridization(ISH)：t

9、heexpressionofNMP·9mRNAinarterioleswallinhypoxicconditionswassignificantlyhigherthanthenormalgroup(P

10、rmalgroup(p