大海马甾醇提取物对小鼠生殖系统的影响与机理-研究

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1、万方数据浙江工商大学硕士学位论文TheeffectandmechanismofsterolinHippocampusKudaonmicereproductivesystemAbstractobjectives：TheaimofthisstudyistoexaminetheeffectandmechanismofsterolextractinHippocampusKudaonmalemicereproductivesystem，inordertoprovidetheoreticalbasisforus

2、inghippocampusastraditionalChinesemedicine．Methods：1．Threefactorsassociatedwithextractingeffectweredetermined，includingextractingtemperature，extractingtimeandratioofmaterialtoliquid．Sterolinhippocampuswereextractedwithsoxhletapparatus．Thenwedeterminedt

3、heabsorbanceofsampleswiththevanillin-perchloratecolormethodaftersaponification．Thecontentoftotalsterolinthehippocampuswasdeterminedwithastandardofcholester01．Furthermore，weoptimizedtheextractingconditionsoftotalsterolinHippocampuskudabyBox-Behnkenrespo

4、nsesurfacemethod．2．Thepurifiedsamplewasfurtheridentifiedbyhighperformanceliquidchromatography(HPLC)usingwater，acetonitrileandisopropylalcoholaseluent．3．Seventy．twoICRhealthadultmalemiceweredividedintofourIII万方数据浙江工商大学硕士学位论文groupsrandomly．Thecontrolgrou

5、pwastreatedwith0．9％physiologicalsalineintraperitoneally，whilethelowdoseexposuregrouptreatedwith1mg／mLhippocampussterol，themiddledosegroupwith3mg／mL，andthehighdosegroupwith5mg／mLonceadayfor10．20and30daysrespectively．Eachgroupmiceweresacrificedinturnbyce

6、rvicaldislocationatthe10m，20thand30thdayforfurtheranalysisofthechangeoftheeachvisceracoefficient，thespermconcentrationandmovementparametersandlevelsoftestosterone(T)，follicle-stimulatinghormone(FSH)，luteotrophichormone(LH)，HDL-candLDL—Cofthemicesera．La

7、ctatedehydrogenase(LDH)，acidphosphatase(ACP)andalkalinephosphatase(AKP)intesticularhomogenatewerealsodetectedusingthedeterminationkit．Thechangesoftheorganizationalstructureofmicetestisandkidneywereobservedbylightmicroscope．Thechangesofexpressionlevelso

8、fStAR，P450scc，P45017andP450aromgenesinmicetestisweredetectedbyquantitativeRT-PCRtechnology．Results：1WeobtainedthedatamodelbytheresponsesurfaceanalysisTheamountoftotalhippocampussterolextraction(mg／g)=4．85+O．38xA+0．74×B+O．62xC+0．32×AxB+O