超高效液相色谱串联多级质谱法分离与鉴定油茶籽中的化学成分

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1、北京化工大学硕士学位论文1．Atotalof60compoundswereidentifiedunambiguouslyortentativelyincluding8flavones，7peptides，36triterpenoidsaponins，6procyanidinesand3othercompounds．Amongthesecompounds，35triterpenoidsaponinswereidentifiedasnovelsaponins．Additionally,aflavone，allthepeptidesand4procyanidineswererepor

2、tedingenusCamelliaforthefirsttime．2．Thefragmentationpathwaysofteasaponinsinnegativeionmodeweresystemicallyinvestigatedforthefirsttime，andanewstrategyforrapidcharacterizationofsaponinswasdeveloped．Thesaponinscouldbeidentifiedasthreeparts：acylgroups，glycosides，andaglycones．Firstly,fortheacylgr

3、oups，whetherthesugarresidueionatm／z451orrn／z481wasexistornotcoulddistinguishnormalanddesacyl—saponins．Secondly，theacylgroupandglycosidecouldbededucedfromlossofmonosaccharideresiduessuccessively．Thelastpartwasaglycones．Fordesacyl-saponins，theformulaforaglyconescouldbededucedfromthe【Aglycone-H

4、】一ion，andfornormalsaponins，itcouldbecalculated．3．Thefragmentationbehaviorindifferentcollisionmodes(HCDandCID)werecomparedandevaluated．TheresultshowedthatHCDmodedidn’tsuitfortheidentificationofsaponins，butcontributetocharacterizeflavonesandpeptides。Moreover,HCDmodewasmoreeffectiveonprocyanidi

5、nesthanCIDmode4．Thechemicalconstituentsinthetestawereestimated，anditdemonstratedthattestahadsameflavonesandpeptidesasfruitsdid．However,inteaseedfruit，thespeciesofsaponinswerelessthanthatoftesta(4kinds)．Finally,procyanidinesandcoffinewerenotdetected．Thisstudystatedpotentialdevelopmentvaluesin

6、thetestaofCamellia17摘要oleifera，andprovidedallexampleinidentificationofchemicalconstituentsrapidlyinsamiliarplants．KEYWORDS：CamelliaD红膨raseed，flavones，cyclopeptides，triterpenoidsaponins，procyanidines，LC-MSV北京化工大学硕士学位论文V目录第一章绪论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯11．1茶籽的研究概况⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．11．

7、1．1概述⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．11．1．2茶籽中生物活性成分的研究进展⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯11．2液质联用技术⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯181．2．1液质联用接口技术的发展⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯181．2．2超高效液相色谱技术的发展⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯191．2．3高分辨质谱技术的发展⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．1