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1、中药白头翁对体外大肠杆菌生物膜抑制作用探究［摘要］目的在①30培养皿和96孔板表面建立大肠杆菌生物被膜(E.colibiofilm,EBF),形成体外模型,并开展白头翁对EBF抑制作用的初步研究。方法选取临床分离的大肠杆菌菌株，在①30培养皿和96孔板中分别复制体外EBF模型，将白头翁作用于EBF体外模型，分别采用银染法和MTT法考察白头翁对EBF的影响。结果①30培养皿中，表面观察到黑色呈棉絮状的膜样物，空白组没有此样物质；96孔板中，模型组的0D值为0.079,空白组的0D值为1.548；白头翁醇提组抑制率为25.79%,白头翁水提组抑制
2、率为53.11%；750ug/mL的白头翁对大肠杆菌生物被膜有最佳抑制作用，抑制率为55.17%；药物作用12h后开始白头翁组的EBF明显少于空白对照组，24h效果最好。结论①30培养皿和96孔板表面可以形成大肠杆菌生物被膜体外模型,白头翁可以抑制和破坏早期及成熟的细菌生物膜，且其抑制作用表现出了一定的量效关系。［关键词］大肠杆菌；生物被膜；MTT法；银染法；白头翁［中图分类号］S853.74［文献标识码］B［文章编号］2095-0616(2013)24-20-03Establishmentofinvitromodelforescherich
3、iacolibiofilmandpreliminarystudyinhibitoryeffectofPulsafiliaonthebiofilmYAOYulBAOYongruil,2MENGXianshengl,2WANGShuail,21.LiaoningUniversityofTraditionalChineseMedicine,Dalian116600,China；2.LiaoningModernTraditionalChineseMedicineResearchandEngineeringLaboratory,Dalian116600
4、,China[Abstract]ObjectiveToestablishinvitromodelofescherichiacolibiofilm(EBF)formatedon①30pettidishesand96~wellplates，andinvestigatetheinhibitoryeffectofPulsafiliaontheEBF.MethodsAtthe①30petridishesand96-wellplatesformdtheinvitromodelofEBF,UsetheinhibitoryeffectofPulsafilia
5、onEBFastheexaminationindicator,MTTassaywasusedtodetectthebestextractionsolventandthebestinhibitrate,anddrawedthegrowhcurve；SilverstainingmethodwasusedtoobservethemorphologyofEBFin①30petridishes・ResuItsTherewereblackstainedcottonwoolmembrane-likematerialappearedonthesurfaceo
6、f①30petridisheswithEscherichiacoli,buttheblankgroupdidn，thave・In96-wellplates，theODvaluesofmodelgroupwas1.548,blankgroupwas0.079；TheinhibiteffectofPulsafiliawater-extractionwas25.79%,TheinhibiteffectofPulsafiliaalcohol-extractionwas53.11%.WhentheconcentrationofPulsafiliawas
7、750ug/mL,thebestinhibitionratewas55.17%.PulsafillaactedonEBF12hourslater,theEBFindruggroupwaslessthanthatofblankgroupdistinctly,thebesteffectwas24h.ConclusionEBFofEscherichiacolicanbeestablishedonthesurfaceof①30petridishesand96-wellplates,Pulsafiliacaninhibitanddestroytheea
8、rlyandmatureEBF,andtheinhibitoryeffectshowsaquantity-activityrelationship・[Keyword
