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1、POGA0112/8/058:41AMPageiPrinciplesofGeneManipulationandGenomics··POGA0112/8/058:41AMPageii··POGA0112/8/058:41AMPageiiiPrinciplesofGeneManipulationandGenomicsSEVENTHEDITIONS.B.PrimroseandR.M.Twyman··POGA0112/8/058:41AMPageiv©2006BlackwellPublishingBLACKWELLPUBLISHI
2、NG350MainStreet,Malden,MA02148-5020,USA9600GarsingtonRoad,OxfordOX42DQ,UK550SwanstonStreet,Carlton,Victoria3053,AustraliaTherightsofSandyPrimroseandRichardTwymantobeidentifiedastheAuthorsofthisWorkhavebeenassertedinaccordancewiththeUKCopyright,Designs,andPatentsAct
3、1988.Allrightsreserved.Nopartofthispublicationmaybereproduced,storedinaretrievalsystem,ortransmitted,inanyformorbyanymeans,electronic,mechanical,photocopying,recordingorotherwise,exceptaspermittedbytheUKCopyright,Designs,andPatentsAct1988,withoutthepriorpermission
4、ofthepublisher.Thismaterialwasoriginallypublishedintwoseparatevolumes:PrinciplesofGeneManipulation,6thedition(2001)andPrinciplesofGeneticAnalysisandGenomics,3rdedition(2003).Firstpublished1980Secondeditionpublished1981Thirdeditionpublished1985Fourtheditionpublishe
5、d1989Fiftheditionpublished1994Sixtheditionpublished2001Seventheditionpublished200612006LibraryofCongressCataloging-in-PublicationDataPrimrose,S.B.Principlesofgenemanipulationandgenomics/S.B.PrimroseandR.M.Twyman.—7thed.p.;cm.Rev.ed.of:Principlesofgenemanipulation.
6、6thed.2001and:Principlesofgenomeanalysisandgenomics/SandyB.Primrose,RichardM.Twyman.3rded.2003.Includesbibliographicalreferencesandindex.ISBN1-4051-3544-1(pbk.:alk.paper)1.Geneticengineering.2.Genomics.3.Genemapping.4.Nucleotidesequence.[DNLM:1.GeneticEngineering.
7、2.BaseSequence.3.ChromosomeMapping.4.DNA,Recombinant.5.Genomics.QH442P952pa2006]I.Twyman,RichardM.II.Primrose,S.B.Principlesofgenemanipulation.III.Primrose,S.B.Principlesofgenomeanalysisandgenomics.IV.Title.QH442.O422006660.6′5—dc222005018202Acataloguerecordforthi
8、stitleisavailablefromtheBritishLibrary.Setin10/12.5ptPhotinabyGraphicraftLimited,HongKongPrintedandboundintheUnitedKingdombyTJInternational,Padstow,Corn
