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1、METHODSINMOLECULARBIOLOGY™SeriesEditorJohnM.WalkerSchoolofLifeSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForfurthervolumes:http://www.springer.com/series/7651wwwwwwwwwwSyntheticGeneNetworksMethodsandProtocolsEditedbyWilfriedWeberBIOSSCentreforBiologicalSignallingStudies,Uni
2、versityofFreiburg,Freiburg,GermanyDepartmentofBiosystemsScienceandEngineering,ETHZurich,Basel,SwitzerlandMartinFusseneggerDepartmentofBiosystemsScienceandEngineering,ETHZurich,UniversityofBasel,Basel,SwitzerlandEditorsWilfriedWeberMartinFusseneggerBIOSSCentreforBiologicalDepartmentofBiosystemsSci
3、enceSignallingStudiesandEngineeringUniversityofFreiburgFreiburg,GermanyETHZurichUniversityofBaselandBasel,SwitzerlandDepartmentofBiosystemsSciencefussenegger@bsse.ethz.chandEngineeringETHZurichBasel,Switzerlandwilfried.weber@biologie.uni-freiburg.deISSN1064-3745e-ISSN1940-6029ISBN978-1-61779-411-
4、7e-ISBN978-1-61779-412-4DOI10.1007/978-1-61779-412-4SpringerNewYorkDordrechtHeidelbergLondonLibraryofCongressControlNumber:2011940329©SpringerScience+BusinessMedia,LLC2012Allrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress,c/oSp
5、ringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisfo
6、rbidden.Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.Printedonacid-freepaperHumanaPressispartofSpringerScience+BusinessMedia(www.springer.com)Pref
7、aceTherapidexpansionofsyntheticbiologyshowingexponentiallygrowingpublicationnum-bersisbasedonthedesignandconstructionofsyntheticgenenetworksopeningmanynewavenuesinfundamentalandappliedresearch.Inthisbook,weprovidecompr