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1、葛根素对急性缺血再灌流损伤肾脏的保护作用Theprotectiveeffectofguggeninontherenalprotectionofacuteischemia2011-09-15jiangdenglinisinhertimePuerarin(universallaw)fromtraditionalChinesemedicine(TCM)inwildradixpuerariaeflavonoidsextract,separationandpurificationofaninjection,alargen
2、umberofanimalexperimentsandclinicalresearch:puerarincanimprovecardiac,cerebralischemicdiseasesanditseffectonthemicrocirculation[1];However,theactiveeffectsofthelipidperoxidationprocessandNa+-k+-atpasewerenotreported.Thisexperimenttoestablishacuteischemiainth
3、eratkidneyperfusionmodel,observationofpuerarinonacuteischemicrenalperfusionSDratsagainformfunctionandrenaltissuemalondialdehyde(MDA)andtheinfluenceofNa+-k+ATPaseactivity,inordertoinvestigatepuerarinonacuteischemiaandperfusiondamagerenalfunctionprotectionandm
4、echanism.One,materialandmethod1.Experimentalanimal:atotalof18maleSDrats,weight(180~200g),choose8weeks,providedbyexperimentalanimalcenteroffujianmedicaluniversity,wererandomlydividedintocontrolgroupandthecontrolgroupandtreatmentgroup.2.Model:ratsbypentobarbit
5、alsodium(40mg/kg)freebilateralkidneysafterintraperitonealinjectionofanesthesia,silverclipblockrightrenalarteriesandveinsletgoafter30minutes,observetherightkidneyhyperemia,ligationofleftrenalarteriesdndveins,excisionoftheleftkidney.Surgicalgroup:dissociatedbi
6、lateralkidney,expose30minutestobesuture,donotbetreated・Treatmentgroup:renalischemiabeforetailintravenousinjectionpuerarin(sino-foreignjointventureofyantaizhongeepharmaceuticalco.,LTD15mg/kg),ischemia30minutesafterintravenousadditionalsamedosedrugs,accordingt
7、o30mg/kgdoseafterintraperitonealinjectiononceeverysixhours・Controlgroup:usingthesamemethodofinjectionforinjectionofsaline,threegroupsofratswereexecutedafteranother48hoursofperfusion・TheeffectsofagrogeninonrenalacuteischemiareinfecationofbloodScr,renaltissueM
8、DAandNa+-k+-atpaseDon，tcountbloodcreatinine(mumol/L)(nmol/mg)(mu,PI*mg*pr-1/10min)Thesurgicalgroupwas656・12plusorminus8.524.81+/-0.5238.12+/—3.21Treatmentgroup696.12++5.02++0.35*34.03++2.39Theco
