rhegf在食管黏膜损伤部位对mscs招募及定居影响的研究

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1、rhEGF在食管黏膜损伤部位对MSCs招募及定居影响的研究哈伟杰温剑虎【摘要】目的：观察rhEGF在食管黏膜损伤部位对MSCs招募及定居的影响。方法：实验大鼠随机分组，MSCs经DAPI标记后移植入实验大鼠体内．每组采用尾静脉和局部注射2种移植方式,2周后取食管组织切片，激光共聚焦显微镜下观察植入细胞的招募及定居情况。结果：局部移植组：食管损伤且给予rhEGF组可见较多量DAPI标记的细胞,未给予rhEGF的损伤组见少量DAPI标记的细胞,二者比较有统计学意义，与正常对照组比较有统计学意义；尾静脉移植组：仅有极少量DAPI标记的细胞.

2、损伤且给予rhEGF组与正常照组间比较有统计学意义，未给予rhEGF的与正常对照组比较无统计学意义。结论：MSCs可在损伤的大鼠食管组织中被招募并定居，局部移植与尾静脉移植途径有差别，rhEGF对于MSCs在损伤的大鼠食管组织招募及定居具有一定的影响【关键词】骨髓；间充质干细胞；食管损伤；招募；定居。TheinfluenceoftheRhEGFontherecruitingandsettlingoftheMSCsoninjuredpartoftheesophagealmucosalHaweijieWenjianhuDepartment

3、ofCardiothoracicSurgery,TheFirstAffiliatedHospital,ChongqingMedicalUniversity,Chongqing400016,ChinaCorrespondingauthor:Wenjianhu,Email：tiger001@163.com[Abstract] ObjectiveToobservetheinfluenceoftheRhEGFontherecruitingandsettlingoftheMSCsoninjuredpartoftheesophagealmucos

4、al.MethodTheSDratswereinvestigatedanddividedintogroupsrandomly.SDratMSCsmarkedbyDAPIwastransplantedintotheexperimentalgroupsandcontrolgroupsratsbytailveininjectionandlocalinjectionrespectively.TheexperimentgroupsweregivenrhEGF.After2week,theesophagealtissuesweretakentoo

5、bservetherecruitmentandsettlementofimplantedcellswiththelaserscanningmicroscopesituation.ResultThegroupinjectedlocallyandgivenrhEGFcouldbeseen more DAPIlabeledcells,thegroupinjectedlocallybutwithoutrhEGFcanbeseen DAPIlesslabeledcells.Therewassignificantdifferencebetween

6、thesetwogroups.Thereweresignificantdifferencesbetweenthenormalgroupandinjectedlocallygroup,bothingivenrhEGFandwithoutrhEGFrats.TherewerefewDAPIlabeledcellstobeobservedintailveingraftgroup.TherewassignificantdifferencebetweentheinjectedlocallyandgivenrhEGFgroupandnormalg

7、roup,andtherewasnosignificantdifferencebetweenthetailveininjectedbutwithoutrhEGFgroupandnormalgroup.ConclusionMSCscanberecruitedandsettledinratsdamage esophagustissue.Thereweredifferentresultsbetweentailveinandlocalinjection.rhEGFcouldinfluence therecruitmentandsettleme

8、ntofMSCsinratinjuryesophagus.[Keywords]bonemarrow—derivedmesenchymalstemcellesophagealinjury  recruitmentsettl