沙棘叶中多糖的初步研究多杰才旦

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1、沙棘叶中多糖的初步研究分类：毕业论文200706摘要本实验首次探讨了沙棘叶中多糖的提取分离，测定其多糖的百分含量。首先用蒸馏水清洗干叶，以洗掉尘土、浮渣，去掉粗梗，剪细。适量石油醚反复脱脂，滤渣自然挥干，再用常温水浸泡一次，80℃热水浸泡两次，以提取水溶性物质，然后合并所有水提液，采用旋转蒸发仪在适宜条件（t＝50℃，抽真空0.09Mpa）下浓缩溶液至适宜体积，sevage法反复操作去蛋白，最后用95％的乙醇使溶液达到不同的浓度而沉淀出多糖，优选出沉淀沙棘多糖的最优醇浓度。0－5℃冰箱中放置48

2、小时后，过滤时用无水乙醇，丙酮洗数次，真空抽干。粗产品重新溶解于适量水中，然后通过大孔树脂进行纯化，蒸馏水洗脱，洗脱液在PH值为8时，用20％的H2O2，50℃以下保温2h脱色，以保证产品的颜色，最后用95％的乙醇使溶液醇浓度达到80％以析出多糖，重复上面的析糖操作，最后保存产品于干燥处。用D（＋）—葡萄糖作标准，苯酚－硫酸法制备标准曲线，求出线性回归方程，从而得到粗多糖的含量，由此也可以算出沙棘叶中多糖的百分含量。然后进行TLC法初步定性确定多糖成分，再进行核磁共振定性分析，即可检验其准确成分

3、。关键词沙棘；多糖；提取；脱蛋白；AB－8大孔树脂 AbstractTheexperimentisthefirsttoresearchtheHippohaerhamnoidesLleaves’polysaccharides.ThepoposeoftheexperimentistakingthepolysaccharidesfromtheHippohaerhamnoidesLleaves.Firstwashtheleavesthroughthewatertocleanthedirtaway,the

4、nusepetroleumethertodegreasetheleavesmanytimes,extractthewater-solublematerialthroughhotwaterof80℃,utilizingtherevolval-evaporationunderthecondition(t=50℃,vacuum0.09Mpa)toconcentratetheaquatotheproperphysicalvolume.Isolationandcompositionofpolysaccha

5、ridesfromtheaqua,alcoholprecipitatebyhotwaterextractionandremoveproteinfrompolysaccharidesby"sevag",inordertogetoptimumalcoholconcentrationfromprecipitatingHippohaerhamnoidesLpolysaccharides.AndalsotrypolysaccharidespurifiedbyAB-8MacfofeticulafResint

6、oachievegoodresults.Finallysumuptheoptimumtechnologyofextractionandpurificationpolysaccharidesfromtheleaves.thenlyingintherefrigerator(0-5℃)twodays,fitratedbyalcohol,acetone,morethanthreetimes.Dissolveintotheproperwateragain;throughAB－8Macfofeticulaf

7、Resin,watercleanse;addthesodiumhydroxidetomakethewater’sPH8,usethehydrogenperoxide(20%),below50℃preservation2hourstoensurethecoloroftheproducts.;thenalcoholprecipitatingagain.WiththeD(+)-theglucoseasthestandardtofigureoutthestandardcurveinthewayof“ph

8、enol-sulfuricacid”,thusobtainingthecontainmentofthecudepolysaccharides.Thismethodwillincreasetheproductionofpolysaccharides,andtheuseofAB－8MacfofeticulafResinwillenhancethepurityoftheproductsandreducethecost. KeywordsHippohaerhamnoidesL;Polysaccharid