高浓度甘油发酵1,3-丙二醇菌株的筛选及其发酵动力学研究

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1、上海师范大学硕士学位论文AstractAbstract1,3-propanediol(1．3-PD)isacknowledgedasoneofsixnewpetrochemicalproductscurremlyintheworld．Itsmainfunctionisanimportantmonomertosynthesizeanewtypeofpolyester,polyether,polyurethane．1，3一Propanediolisproducedbytwoways，chemicalsynthesisandmicrobialconversi

2、on．Ithasadvantagesforhigh-convertingenzymeandexpressionefficiency．Producing1,3-propanediolbymicrobialfermentationhavemanyobviousadvantagesandbecomethefocusesofresearch．Thisarticlemadethefollowingfindings：1，Screeningtotoleratehighconcentrationsofglycerol1,3一propanediolproducingst

3、rainScreeningofhighconcentrationsofglyceroltolerantstrainsimprovefromafmalglycerolconcentrationof10％to30％gradually，outofthelowstraintoleranceofglycerol，fromtheoutsetahightolerancetostrainglycerine．Furthermoreinquiriesmayutilizeglycerolmicrobialsynthesisproduct，findamicroorganism

4、fromglycerolandthreefoundastraincapableofproducing1,3-propanediol(1abeledD1)，andthusitsphysiologicalandbiochemicalexperimentsand16SrRNAhomologyphylogeneticanalysistodeterminethestatusofthisstraincametotheconclusionthatitisKlebsieIlaconclusions．2，optimizedproductionstrainsmediumc

5、ompositionandmaintechnicalparametersPreliminaryexperimentsusingsinglefactoroptimizationKlebsiellagrowthmediumcomponentsandfermentationmediumconditionswereinvestigatedindifferentcomponentsofthestarterculturebacteriacultureandfermentationoftheproduct，andtheuseofresponsesurfaceexpe

6、rimentaldesignoptimizationthemediumcomposition．Optimizationofgrowthmediumwere：KH2P041．49g／L，K2HP043．4g／L，(NH4)2S043g／L，CaCl20．02g／L，FeS041．3g／L，yeastextract，1g／L，glycerol50．3g／L，fortheoptimizationoffermentationmedium"KH2P041．5g／L，K2HP043．4g／L，(NH4)28043g／L，CaCl20．047g／L，FeS040．0

7、1g／L，yeastextract1．2g／L，glycerol224．229／L．Fermentationoptimizedthanthepreviousoptimization1,3一propanediolproductionincreasedby58．92％．ItWasalsotheuseoforthogonalexperimenttooptimizetheseedfermentationprocessparameterswereinvestigatedtheeffectsofpH，temperatureandshakingspeedandoth

8、erparametersofthefermentationconditions．Theopti