核糖对应激性胃溃疡和心肌细胞缺氧复氧损伤的保护作

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1、优秀毕业论文AbstractStressgastriculcerhasseriouslyeffectsonthequalityofpeople’Slife．Thedrugonthemarketismetabolizedbyliversandexcretedbykidneysalongwithincreasingburdenofliversandkidneys，thenaffectthefunctiononthem．Inordertofindoutamaterialwhichhasnoburdenofbodyfor

2、thetreatmentofgastriculcer,thisarticleinvestigatedtheprotectiveeffectofD．．riboseonstressgastriculcer．TheeffectsofD--riboseonantioxidationeffectofmyocardialcellswerealsostudiedatthecellularlevel．Thewater-immersionrestraintstressinducedgastriculcermodelwasestab

3、lishedofSDrats．TheSODactivityofserumandgastricmucosatissue，MDAcontentofserumandgastricmucosatissueandMPOactivityofgastricmucosatissueweredetectedbyusingxanthineoxidasemethod，thiobarbituricacidmethodandELISArespectively．ThegeneexpressionlevelofIGF．．1andTIMP．．2

4、ofgastricmucosatissuewasdeterminedbyrealtimequantitativePCR．Primarymyocardialcellsweresuccessfullyculturedinvitro．Thegrowthstateofcardiacmusclecellswasobservedusinginvertedphasecontrastmicroscope．TheactivityofmyocardialcellsandtoxicityofD—ribosewereinvestigae

5、dbyexclusionmethod．TheSODactivityofmyocardialcellswasanalyzedbyxanthineoxidasemethod．TheresultsshowedthatD—ribosesignificantlyincreasedtheSODactivity,decreasedtheMDAcontentandMPOactivity,down—regulatedthegeneexpressionlevelofIGF一1，andupregulatedthegeneexpress

6、ionlevelofTIMP一2．D—ribosecouldreduceoxygenfreeradicalgeneration，maintainoxidationandantioxidationbalance，avoidtheoccurrenceofoxidativestress，neutrophilinfiltrationandinflammatoryreaction，andmaintaintheintegrityofthestructureandfunctionofthecell．Therefore，D—ri

7、bosemayhaveafunctioninthepreventionandtreatmentofgastriculcer．D·ribosehadnon—toxiceffectonmyocardialcells．TheconcentrationofD．ribosesolutionin5me／mE，10me／mEand15me／mEhadthestrongestantioxidanteffectonthecells．Hypoxia／reoxygenationmodelofmyocardialcellwasestab

8、lishedsuccessfullyinvitrotosimulatetheischemiareperfusioninjuryofmyocardialcell．D．ribosesignificantlyincreasedSODactivityandimprovedtheantioxidationlevel．D-ribosemayhaveafunctionintheprev