玉米螟生防工程菌株构建的分析

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1、AbstractAbstractAsiancornborer(Ostriniafurnacalis)，heavilydestructionandwidelydistribution,isoneofthemostimportantagriculturalpestintheworld．Becauseofitsrapidproduction,moregenerationandhavingastrongerpesticidesresistance，thechemicalcontrolontheAsiancornborerfacesmoreandmoredifficultie

2、s．So，findingahigh-efficient,safeandenvironment-friendlysubstituteofpesticidebecomesaproblemneedtOberesolved．Inthel沱Seal'ch,bytakingeomandcornborer嬲researchobjects，60differentiallyexpressedproteinsrespondedtoMJAtreatmentwereidentifiedbythemear塔of2DjandMSbasedonanalyzingthemaizeresistanc

3、erespondinducedbyMJA．Theirfunctionswereconfirmedaccordingtotheproteindatabasecorresponded．Thediscoveredproteinsplayimportantrolesintheplantsdefenseresponse．Thoselaidafoundationonconstitutingbio-controlengineeringstrainforaSiancornborer．Fourlimportantplantdefenseregulatedproteinswerecho

4、senandtheiranti．insectactivitiesiwereanalyzed．ProkaryoticexpressionvectorswerebuiltbythepETsystem，andtherecombinantproteinsWel"ealsoinducedexpressedsuccessfully．Theinfluencesof‘：recombinantproteins’biologicalactivitiesonAsiancornborerwerealsotestedbybioaS娜Ther黜mbin瓤p腻iIlseXprcssedby姚pE

5、他8棚Ⅸ锄dp肌8a．PRP移hadsignificanteffectsonthedevelopmentofAsianeomborer,andtheinhibitionrates‘’ofgrowthwere34．14％and30．33％respectively．AlthoughtherecombinantproteinexpressedbypET28a-GRBPhadnosignificationeffectonthedevelopmentofAsiancomborer,ithadasignificationeffectonthepupaweight．Thoseim

6、plythattheproteinsindentifiedmaybeanti—insectproteins、ⅣitllhighactivitiesfromMaize．Glutathione-S-transferasea陀importantdetoxifyingenzymesininsect．Ithas锄effectofmetabolismtOxiccompoundsinboththeinsectbodiesandenvironment,innordertoenhancethebody’Sresistantability．AfulllengthGSTeDNAWaSis

7、olatedfromthemidgutofAsianeomborer,namedOfGST,byRT-PCRandRACE，andthebioinformatiesanalysiswereconductedafterthat．BaSedonthose，bulittheprokaryotieexpressionvector-andperformedthepolyelonaiantibodybythepurifiedrecombinantproteins．Western。blotshowedthattheexpressionlevelofGSTincreaSedaf