原发性干燥综合征外周血cd4+t细胞dna甲基化异常的分析

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1、硕士学位论文英文摘要ABSTRACTObjective:PrimarySj6gren’5syndrome(PSS)15anautoimmunediseaseeharacterizedbylymPhoeytieinfiltratlonandtheProduetionofautoantibodies，leadingtothedestruetionoflaerimalandsalivaryglands.However，verylittle15知ownaboutthePathogenesisofthisdisorder.Inreeentyears，evidencelinkinga

2、berrantDNAmethylationwithregulationofgenesexPressioninTeellsandautoimmunediseaseshasbeenaecumulating.Forinstance，genome一widedeereasesindeoxy-methyleytosineandhyPomet勿lationofsPeeificgenes，suehasCDlla(IT(训L)，CD70(刀叨邪伊，乃，CD4OLigand(刀吓万石万)andPerforin(尸尺月)，havebeeneonfirmedinsystemieluPuse州he

3、matosus(SLE).OvereXPressionofthesegeneseanindueeautoreactiveTcells，exeessiveBeellstlmulationandmacroPhagekilling，andeontributestothePathogenesisinSLE.Inthisstudy，weinvestigatetheexPressionlevelsandmethylationPattemsofsomeautoimmune一relatedmet勿lationsensitivegenesinCD4+TeellsfromPSSPatient

4、sandhealthyeontrols，aimingtodiscovertheaberrantePigenetieregulationsinPSSandoPenuPnewavenuesforrevealingthePathogenesisofPSS.Methods:PBMCs(PeriPheralbloodmononueleareells)wereisolatedfromthePeriPheralvenousbloodof17PSSPatienisand14healthydonorsbydensitygradienicentrifugation，andtheflowcyt

5、ometrywasusedtodeteettheexPressionlevelsofCD1la，CD70andCD40LinCD4+Teells.CD4+TeellswereisolatedfromthePBMCusingmagneticcellseParationteehnique，thenthemRNAlevelsofCD70andPerforininCD4+Teellsweremeasuredbyreal一timequantitatiVereversetranscriPtase一Polymeraseehainreaction(real一timeRl’-PCR).Pe

6、rforinProteinwasanalysedbyw七stemblot.Bisulfitesequeneingwasusedtodeterminethemethylationstatusoftheregulatorysequeneeinwhieh111硕士学位论文英文摘要genetheeXPressionaresignifieantlydifferenee.Results:CD70mRNAandProteinIevelsaresignificantlyinereased(P=0.001，P=0.003:resPeetively)inCD4+TeellsofPSSPati

7、eniseomParedtoeontrols.PerforinmRNAlevelinCD4+Teells15signifieantlyinereased(P=0.018)eomParedtotheconirols，butnosignifieantlydifferencewasfoundinProteinlevel.AndtheeXPressionofCD11aandCD40LhavenosignifieantlydiffereneebetweentwogrouPs.TheTNFSF7Promoter15demethylated