ctz癫痫模型与ptz ka模型的比较分析

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1、优秀毕业论文复旦大学硕士学位论文AbstraCtEPilePsy15afrequentlyoeeurredCNSdisease，bynowmostseizureseanbeeontrolledbytreatment.However，weareshortofcomPletelyeffectivetheraPymethodsineontrollingePilePsybutsometimesuseAEDstosuPPresstheseizures.Atthesametime，wehaveknownlittleabou

2、tthemeehanismorPathogenesisofePilePtogenesis，andit15ve叮imPortanttofindouttheereationanddeveloPmentofseizures.SettinguPfastandhigh一effeetiveePilePsymodelseanProvideusbothwaystostudythemeehanismandnewtargetsforAEDssereening.InPresentstudywestudiedanewseizuremo

3、del(indueedbyCTZ)reeentlydeveloPedinourlab，alongwithseveralotherehemieally一indueedaeuteseizuremodelsinrathiPPocamPusinvivo.w亡analysesedandeomParedeharaetersofthesemodelsbyfieldpotential，behavioralandPharmaeologiealteehniques.Inthesemodelswefurtherstudiedsome

4、anti一ePilePtiedrugs’effeet，whiehmightProvidenewtargetsforfutureAEDsstudies.W匕foundthati.e.v.administrationofCTZ(5林mol，5林l)indueedrobustePilePtiformaetivitiesinatime一dePendentmannerstePPingfrommildsPontaneoussPikestohighlysynehronizedePilePtiformbursts.Italso

5、indueedinereasingnumbersofevokedPoPulationsPikefromsinglePeaktomultiPlePeaks.SubeutaneousadministrationofPTZ(383mg/kg)sharedasimilarProgressasCTZexeePtforsomediffereneesinintensityandtimeeourse.KAinducedamuehseverermodelthanCTZdidwhengive1.e.v.withafasterPro

6、gressandhigherintensity.BesidessPontaneoussPikesandbursts，KAalsoindueedrobustePilePtiformaetivitiesofaseizurestateeontaininglong一lastingeontinuouslyfiringsPikesasaeutestatusePilePtieus(ASE).TheseizuresensitivityrankingfromhightolowseemedasKA，PTZandCTZ.Neithe

7、raeutebehavioralnoreleetroPhysiologiealePilePtiformaetivitiesweregeneratedwhenPTZwasgiveni.e.v.atarelativelyhighdose.PharmaeologiealstudyshowedthatCTZandPTZmodel15differentinmeehanism.AdministrationofAlprazolam(o.4mg/kg，i.p.)，ahigh一PoteneybenzodiazePine，effe

8、etivelyinhibitedbothCTZandPTZindueedbursts，buthaslimiteffeetonKAindueedASEstate.Whilenon一eolnPetitiveAMPAreeePtorantagonistGYKI53784(smg/kg，i.v.)onlysuPPressedCTZbutnotPTZinducedburstaetivities.