bsep在辅助生殖技术助孕的icp患者胎盘中的表达及意义

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1、BSEP在捕BJ注趣沐助孕的ICP患者胎盘中的表达及意2018-03-2712:00:30屮国现代医牛2018年3期刘林丽张良K朱思远齐碧如黄勉［摘耍］鬥的探討胆盐输山泵(BSEP)在ART助孕和正常受孕妊娠期肝内胆汁淤积症(ICP)患者胎盘组织中的表达及意义。方法采用Westernblot和RT-PCR方法检测我院收治的15例自然受孕ICP患者(ICP组)、15例ART助孕ICP患者(ART组)及9例正常妊娠患者(对照组)胎盘组织屮BSEP蛋白和mRNA的表达怙况，分析三组ICP胎盘屮BSEP表达的差异。结果Westernblot和RT-PCR法显示BSEP蛋白

2、和mRNA在自然受孕ICP胎盘巾表达分别为(0.443±0.102)和(8.139±4.490)，在ART来源胎盘中表达分别为(0.490±0.117)和(9.992±5.253),而正常妊娠胎盘组织中表达明显减少(0.161±0.013)和(1.038±0.271),ICP组和ART组差异无明显差异(P>0.05),而这两组与对照组比较差异有统计学意义(P<0.001)。结论BSEP蛋白在正常妊娠胎盘组织屮微显表达，而在ICP患者胎盘组织屮商表达，可能与ICP的发病有关，但向然受孕ICP胎盘和ART助孕ICP胎盘中BSEP蛋白无差异，提示ART助孕并不增加子代妊

3、娠期ICP的发病风险。［关键词］辅助生殖技术；蛋白质印迹法；奸娠期肝内肌汁淤积症；反转录•聚合酶链反应；月R盐输出泵［屮图分类号］R715.5［文献标识码］A［文章编号］1673-9701(2018)03-0008-04SignificanceandexpressionofBSEPinplacentaofICPpatientstreatedwithassistedreproductivetechnologyLIULinliZHANGLiangchenZHUSiyuanQlBiruHUANGMianDepartmentofObstetricsandGynecolog

4、y，FuzhouFirstAffiliatedHospitalofFujianMedicalUniversity,Fuzhou350004,China［Abstract］ObjectiveToexplorethesignificanceandtheexpressionofbilesaltexportpump(BSEP)inplacentaofpatientswithintrahepaticcholestasisofpregnancy(ICP)ofnaturalconceptionorpregnancythroughARTtreatment.MethodsTheex

5、pressionofBSEPproteionandmRNAinplacentaof15ICPpatientsofnaturalconception(ICPgroup),15ICPpatientstreatedwithART(ARTgroup)and9normalcasesofnaturalconception(controlgroup)attendedourhospitalwasdetectedbyWesternblotandRT-PCR.ThedifferenceofBSEPexpressioninthreegroupsofICPplacentawasanaly

6、zed.ResultsTheWesternblotandRT-PCRshowedthattheexpressionofBSEPproteinandmRNAinICPplacentaofnaturalconceptionwas(0.443±0.102)and(8.139±4.490),respectively.TheexpressionintheplacentaofARTgroupwas(0.490±0.117)and(9.992±5.253)，respectively.Theexpressionintheplacentaofcontrolgroupwassigni

7、ficantless,whichwas(0.161±0.013)and(1.038±0.271),respectively.TherewasnosignificantdifferencebetweenICPgroupandARTgroup(P>0.05)，butbothofthemshowedstatisticallysignificantdifferencecomparedtocontrolgroup(P<0.001).ConclusionTraceexpressionofBSEPproteinwasfoundinplacentatissueofnormalpr

8、egnan