toll样受体3在大鼠急性心肌梗死中的作用机制

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1、Toll样辦3献鼠急用刪2018-03-2001:26:12中国现代医生2018年1期王媛媛曹建邓莉［摘耍］鬥的研宂Toll样受体3(TLR3)在大鼠心肌梗死屮的表达及作用机制。方法制备TLR3基因敲除大鼠及野生型大鼠，采用球囊结扎冠脉方法制作心肌梗死动物模型。随机分为野生型假手术组(n=15)、野生型心肌梗死组(n=15)、(TLR3-/-)假手术组(n=15)、(TLR3-/-)心肌梗死组(n=15)。检测心肌组织肌酸激酶同工酶(CK-MB)、肌钙蛋白cTnl含虽；通过Westernblot

2、法检测Toll样受体3、NF-kB表达。结果与野生型心肌梗死组大鼠比较，(TLR3-/-)心肌梗死組反映心肌损伤的CK-MB、cTnl减低；NF-kB表达降低；各组间差异均有统计学意义(P<0.05)。结论TLR3S因缺失在急性心肌梗死中A•有心脏保护作用，其保护机制与下调NF-kB衣达，减轻心肌梗死炎症反应有关。［关键词］TOLL样受体3;急性心肌梗死；核因子-kB;棊因敲除［中图分类号］R541;R392［文献标识码】A［文章编号］1673-9701(2018)01-0036-03Mecha

3、nismofToll-likereceptor3inacutemyocardialinfarctioninratsWANGYuanyuanlCAOJian2DENGLi11.DepartmentofCardiology，theThirdHospitalofNanchang，Nanchang330009，China；2.DepartmentofAnesthesiology,theSecondAffiliatedHospitalofNanchangUniversity,Nanchang，330006

4、，China［Abstract］ObjectiveTostudytheexpressionofToll-likereceptor3(TLR3)inmyocardialinfarctionofratsanditsmechanism.MethodsTLR3geneknockoutratsandwild-typeratswereprepared.Animalmodelsofmyocardialinfarctionwerecreatedbyballoonligationofcoronaryarterie

5、s.Theratswererandomlydividedintowild-typeshamsurgerygroup(n=15),wild-typemyocardialinfarctiongroup(n=15),(TLR3-/-)shamsurgerygroup(n=15),and(TLR3-/-)myocardialinfarctiongroup(n=15).Thecreatinekinase(CK-MB)andtroponincTnlcontentsinmyocardialtissueswer

6、emeasured.Toll-likereceptor3andNF-kBexpressionsweredetectedbyWesternblotmethod.ResultsComparedwiththeratsinthewild-typemyocardialinfarctiongroup，thelevelsofCK-MBandcTnlwhichreflectedmyocardialinjuryinthe(TLR3-/-)myocardialinfarctiongroupweredecreased

7、；NF-kBexpressionwasdecreased；thedifferencesbetweengroupswerestatisticallysignificant(P<0.05).ConclusionTLR3genedeletionhascardioprotectiveeffectsinacutemyocardialinfarction.Itsprotectivemechanismisrelatedtothedown-regulationofNF-kBexpressionandallevi

8、ationofinflammatoryresponseinducedbymyocardialinfarction.［Keywords］TOLL-likereceptor3；Acutemyocardialinfarction；NF-kB；Geneknockout急性心肌梗死是严重危害人类健康的常见疾病之一，研允表明，在心肌梗死沿，心肌组织会分泌大S炎性因子(如白细胞介素)，炎症因子局部W•常表达及不适当堆积可激活细胞毒性T细胞造成心肌细胞的结构和功能障碍，与心室重塑密切相关［1-3］。此外还可引起