碳纳米管阵列修饰的电化学dna传感器用于多药耐药基因的检测

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1、福建医科大学硕士研究生毕业论文groupwasimmobilizedonthepipeendofMWCNTsbyamidebondformationtoconstructtheDNAbiosensor.ChronocoulometrywasemployedtothedetectionofMDR1genebyusingrutheniumhexaammine(RuHex)assignalindicator.Experimentalresultsshowthattheincreasedelectricquantityareproportionaltothelogarithmofth

2、econcentrationofthetargetDNAintherangeof1.0×10-12mol/L~1.0×10-8mol/Lwithadetectionlimitof6.4×10-13mol/L(S/N=3)underoptimalconditions.Inaddition,thebiosensorexhibitsgoodselectivity,acceptablestabilityandreproducibility,whichcanbeusedinthequalitativeandquantitativedeterminationofMDR1gene.Part

3、II:ExperimentalstudiesofAlignedMWCNTsarrays-PDDA-AuNPsmultilayerfilmmodifiedelectrochemicalbiosensorfordetectionofMDR1geneInthispart,auniformandstableelectrochemicalDNAbiosensorwithAlignedMWCNTsarrays-PDDA-AuNPsmultilayerfilmwasconstructedonthegoldelectrodesurface,whichwasbasedonthealignedM

4、WCNTsarraysmodifiedelectrodewebuiltinthefirstpartofthisarticle.Theconstructionofsensinginterfacewasbasedonlayer-by-layerelectrostaticself-assemblytechnique.NegativelychargedcarboxylMWCNTsandgoldnanoparticles(AuNPs)werebridgedbypositivelychargedcationicpolymerpolydiallyldimethylammoniumchlor

5、ide(PDDA)viaelectrostaticforce.5'end-labeledcaptureprobeDNAwithsulfhydrylgroupwasimmobilizedontheAuNPsbyAu-SbondformationtoconstructtheDNAbiosensorforthedetectionofMDR1gene.Experimentalresultsshowthatthismodifiedbiosensorhasgoodstabilityandreproducibility.Furthermore,comparedtothebiosensorw

6、ebuiltinthefirstpart,theDNAhybridizationelectrochemicalsignalsofthismodifiedbiosensorcanbefurtheramplifiedbyAuNPs.Undertheoptimizationofexperimentalconditions,theincreasedelectricquantityareproportionaltothelogarithmoftheconcentrationofthetargetDNAintherangeof5.0×10-13mol/L~1.0×10-8mol/Lwit

7、hadetectionlimitof9.4×10−14mol/L(S/N=3).Italsoshowsexcellentnoiseimmunityinsimulatedhumanserumsampleswhichispossibletoapplyinpracticalsamples.PartIII:ExperimentalstudiesofAlignedMWCNTsarrays-rGO-AuNPsmodifiedelectrochemicalbiosensorfordetectionofMDR1gene